Anti-ATF3 Rabbit Monoclonal Antibody for WB - P18847

Core Product Specifications and Parameters

Biological Background: ATF3 Function and Localization

• ATF3 (Activating Transcription Factor 3) is a basic leucine zipper (bZIP) transcription factor encoded by the ATF3 gene. It binds the cAMP response element (CRE) consensus sequence 5'-GTGACGT[AC][AG]-3' present in many viral and cellular promoters.
• It functions as a dual regulator: repressing transcription from ATF sites by stabilizing inhibitory cofactors at the promoter, and activating transcription by sequestering those cofactors away from promoters.
• Alternative splicing generates multiple isoforms. Isoform 1 acts as a transcriptional repressor, while a stress-induced isoform counteracts this repression, highlighting a dynamic stress response role.
• It is localized to the nucleus, where it orchestrates transcriptional programs in response to various stimuli such as cytokines, growth factors, and DNA damage.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond), which modulate its stability, activity, and protein interactions.
• As a key stress-inducible gene, ATF3 is implicated in processes like apoptosis, cell cycle regulation, and immune responses, linking it to cancer, neurodegeneration, and metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1–77), which may recognize multiple isoforms. Validate specificity in relevant human or mouse cell lines, noting the variable molecular weight (12–21 kDa) often seen in Western blot due to isoform differences and modifications.
• For Western blotting, optimize reducing conditions and acrylamide percentage to resolve small protein bands; consider using 15% gels or gradient gels for better separation.
• In ChIP experiments, crosslinking conditions should be carefully titrated for ATF3's transient promoter occupancy; the antibody may be suitable for enrichment followed by qPCR or sequencing.
• For IP, standard protocols with low-stringency washes may preserve ATF3-interacting complexes.
• ELISA performance can be evaluated by coating recombinant ATF3 protein or lysates; the antibody can potentially be used as a capture or detection reagent in a sandwich immunoassay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with consolidated access to high-quality IVD raw materials. This anti-ATF3 monoclonal antibody supports assay development for cancer, neurology, and stress-related biomarker panels. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and tailored sourcing of rare transcription factor targets, streamlining your journey from concept to clinic.