Anti-ATF2 Rabbit Monoclonal Antibody for WB, IHC-P, IP, ELISA - P15336

Core Product Specifications and Parameters

Biological Background: ATF2 Function and Localization

• ATF2 (Cyclic AMP-dependent transcription factor ATF-2) is also known as CREB-2, HB16, TREB7, and CRE-BP1. It belongs to the ATF/CREB family of transcription factors.
• It functions as a transcriptional activator, binding to CRE (5'-TGACGTCA-3') or AP-1 (5'-TGACTCA-3') consensus sequences, depending on its binding partner.
• ATF2 regulates genes involved in anti-apoptosis, cell growth, and the DNA damage response. It can exhibit both oncogenic and tumor suppressor activities depending on tissue context.
• In the nucleus, ATF2 contributes to global transcription and DNA damage response. In the cytoplasm, it interacts with mitochondrial complexes to perturb membrane potential and promote cell death.
• ATF2 possesses intrinsic histone acetyltransferase (HAT) activity, acetylating histones H2B and H4 in vitro (PubMed:10821277). Related references: PMID:10821277
• The phosphorylated form (mediated by ATM) is involved in ionizing radiation-induced S phase checkpoint control and recruitment of the MRN complex to DNA damage foci.
• ATF2 is localized to the nucleus, cytoplasm, and mitochondrial outer membrane. It is ubiquitously expressed, with higher levels in the brain.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-340) of human ATF2, which encompasses the transcriptional activation domain and zinc finger motif, likely contributing to target specificity.
• For Western blot, the predicted molecular weight is ~55 kDa; consider using appropriate positive and negative controls to validate band identity.
• For IHC-P, optimal antigen retrieval methods should be determined experimentally; tissue samples with known ATF2 expression (e.g., brain) may serve as positive controls.
• For IP, the antibody's performance should be verified in the relevant cell or tissue lysates, ensuring that the target protein is efficiently captured.
• For ELISA, titrate the antibody and optimize coating and detection conditions to achieve the desired sensitivity and specificity.
• Cross-reactivity with mouse and rat is reported, making this antibody suitable for studies in these species; however, validation in the specific experimental context is recommended.

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CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a streamlined one-stop platform for IVD raw material sourcing. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents, we supply the critical components needed for assay development. Our portfolio includes products for transcription regulation and DNA damage response research, including high-quality anti-ATF2 antibodies tailored for multiple applications.