Anti-ATAD3B Polyclonal Antibody for WB, ELISA - Q5T9A4

Core Product Specifications and Parameters

Biological Background: ATAD3B Function and Localization

• ATAD3B (ATPase family AAA domain-containing protein 3B), also known as AAA-TOB3 or TOB3, is a member of the ATPase family AAA domain-containing proteins and is encoded in humans by the ATAD3B gene.
• It may play a role in establishing a mitochondrial network organization typical of stem cells, characterized by reduced mitochondrial metabolism, low mtDNA copies, and fragmented mitochondrial network.
• ATAD3B may act by suppressing ATAD3A function, interfering with ATAD3A interaction with matrix nucleoid complexes.
• The protein localizes to the mitochondrial inner membrane.
• ATAD3B expression tends to be down-regulated in differentiated cells and is re-expressed in pluripotent stem cells or cancer cells (at the protein level).
• Key structural features and post-translational modifications include an AAA+ ATPase domain, coiled coil regions, and sites of acetylation, alongside alternative splicing isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal 1–240 amino acid region of human ATAD3B; consider potential cross‑reactivity with ATAD3A or other AAA‑domain proteins and validate in the relevant sample system.
• For Western blot, a band around 73 kDa is expected; optimize blocking and detection conditions using whole‑cell or mitochondrial‑enriched lysates from stem cells or cancer cell lines.
• In ELISA, titrate the antibody against recombinant ATAD3B or cell lysates to determine a suitable working concentration; note that quantitative performance data is not provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research labs a streamlined path to IVD raw materials, with validated antibodies, bulk ancillary reagents, and rare‑target sourcing capabilities. This anti‑ATAD3B antibody supports mitochondrial and oncology‑related assay development, from concept to clinical application. Partner with us to secure customized polyclonal production, matched antibody pairs, or technical consultation for your next diagnostic project.