Anti-ATAD3A/B Monoclonal Antibody for WB, IHC-P, ELISA - Q9NVI7 / Q5T9A4

Core Product Specifications and Parameters

Biological Background: ATAD3A/B Function and Localization

• ATAD3A is an ATPase family AAA domain-containing protein, existing in multiple isoforms via alternative splicing.
• Localizes to the mitochondrial inner membrane, matrix, and nucleoids, playing a critical role in mitochondrial network organization, metabolism, and cell growth.
  Related references: PMID:17210950, PMID:20154147, PMID:22453275
• May participate in mitochondrial protein synthesis and DNA replication, likely by binding to mitochondrial DNA D-loops and stabilizing nucleoids.
  Related references: PMID:22453275, PMID:17210950
• Required for enhanced cholesterol channeling during hormone-dependent steroidogenesis.
  Related references: PMID:22453275
• Involved in mitochondrial-mediated antiviral innate immunity.
  Related references: PMID:31522117
• Protects mitochondria from PERK-mediated unfolded protein response by inhibiting EIF2AK3/PERK at ER-mitochondria contact sites; this function is independent of its ATPase activity.
  Related references: PMID:39116259
• Participates in mitochondrial DNA damage response, promoting signaling between damaged genomes and the membrane to activate the integrated stress response (ISR).
  Related references: PMID:37832546
• Overexpressed in lung adenocarcinomas at the protein level, suggesting a role in tumorigenesis.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1–240 of human ATAD3A/B, a conserved N-terminal region; this design may support cross-reactivity with both ATAD3A and ATAD3B, as well as with mouse and rat orthologs.
• For Western blot, consider testing multiple tissue lysates (human, mouse, rat) and note the predicted molecular weights of 57, 66, and 71 kDa, which correspond to ATAD3A/B isoforms; validate specificity with appropriate controls.
• In IHC-P, optimize antigen retrieval conditions and validate in tissues known to express ATAD3A, such as lung adenocarcinoma, where overexpression is reported.
• For ELISA, use as a capture or detection antibody in combination with a suitable paired reagent; verify performance under standard assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For mitochondrial function research and cancer biomarker development involving ATAD3A/B, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Count on CamelBio to streamline your IVD development pipeline from discovery to regulatory approval.