Anti-ATAD3A Polyclonal Antibody for WB, IHC-P, ELISA - Q9NVI7

Core Product Specifications and Parameters

Biological Background: ATAD3A Function and Localization

• Essential for mitochondrial network organization, mitochondrial metabolism and cell growth. Related references: PMID:17210950, PMID:20154147, PMID:22453275
• Important for mitochondrial protein synthesis. Related references: PMID:22453275
• Participates in mitochondrial DNA replication and contributes to nucleoid stability by binding D-loops. Related references: PMID:17210950
• Required for enhanced cholesterol channeling in hormone-dependent steroidogenesis. Related references: PMID:22453275
• Involved in mitochondrial-mediated antiviral innate immunity. Related references: PMID:31522117
• Protects mitochondria from PERK-mediated unfolded protein response by inhibiting EIF2AK3/PERK at mitochondria-ER contact sites, independent of ATPase activity. Related references: PMID:39116259
• Promotes mitochondrial DNA damage signaling, activating the integrated stress response (ISR). Related references: PMID:37832546
• Localizes to the mitochondrial inner membrane, matrix, and nucleoid.
• Overexpressed in lung adenocarcinomas at the protein level.
• Contains ATP-binding and coiled-coil domains; undergoes acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• For Western blot (WB), validate using whole-cell lysates from human, mouse, or rat cells; the expected band size is approximately 71 kDa.
• For immunohistochemistry (IHC-P), consider standard antigen retrieval methods such as citrate-EDTA buffer (pH 6.0) and optimize primary antibody concentration.
• For ELISA, coat the antigen at an appropriate concentration and validate specificity against negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutes with a streamlined supply chain for IVD raw materials, including validated anti-ATAD3A polyclonal antibodies for mitochondrial biology research. Our portfolio extends to recombinant antigens, antibody pairs, and bulk ancillary reagents, with dedicated support for custom sourcing of rare target raw materials.