Anti-AT1R/AGTR1 Monoclonal Antibody for WB, ELISA - P30556

Core Product Specifications and Parameters

Biological Background: Type-1 Angiotensin II Receptor Function and Localization

• Type-1 angiotensin II receptor (AT1R), encoded by the AGTR1 gene, is a G protein-coupled receptor (GPCR) with seven transmembrane domains; it is also known as AT1AR, AT1BR, or AT2R1.
• Acts as the primary mediator of angiotensin II effects, functioning in blood pressure regulation and sodium retention by the kidney through coupling to G(q) proteins, activating phospholipase C and elevating cytosolic Ca²⁺ levels. Related references: PMID:15611106 PMID:1567413 PMID:25913193
• Localizes to the cell membrane, consistent with its role as a transmembrane receptor.
• Expressed in liver, lung, adrenal gland, and adrenocortical adenomas.
• Undergoes post-translational modifications including glycosylation, palmitoylation, and phosphorylation; contains disulfide bonds essential for its structure.
• Plays a role in host-virus interaction: during SARS-CoV-2 infection, AT1R recognizes and internalizes the complex of secreted ACE2 and viral spike protein via dynamin-dependent endocytosis.
• Classified under disease variant, reflecting its involvement in cardiovascular and renal pathologies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human AT1R/AGTR1; consider this when designing detection or blocking strategies.
• For Western blot, the expected molecular weight is approximately 41 kDa; use appropriate gel percentage and transfer conditions for a multipass transmembrane protein.
• For ELISA, this antibody can be used in direct or indirect formats; validate in the relevant sample system, such as cell lysates or tissue extracts expressing AT1R.
• Cross-reactivity is confirmed for human; if working with other species, verify sequence homology of the epitope region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated monoclonal antibodies like this anti-AT1R/AGTR1 rabbit mAb to support cardiovascular and infectious disease assay development. Beyond this product, we provide optimized antibody pairs, bulk ancillary reagents, and access to rare target raw materials, streamlining your path from concept to clinic.