Anti-ASUN/INTS13 Rabbit Monoclonal Antibody for WB, ELISA - Q9NVM9

Core Product Specifications and Parameters

Biological Background: ASUN/INTS13 Function and Localization

• Encoded by the INTS13 gene (aliases: ASUN, C12orf11, GCT1), Integrator complex subunit 13 (INTS13) is also known as cell cycle regulator Mat89Bb homolog, germ cell tumor 1, and protein asunder homolog.

• As a component of the integrator complex, INTS13 contributes to transcription termination by RNA polymerase II (Pol II) in the promoter-proximal region of genes. It participates in the quality checkpoint that drives premature termination of unfavorably configured transcripts via CTD dephosphorylation, nascent RNA degradation, and Pol II release. Related references: PMID:38570683, PMID:38823386

• The integrator complex also terminates synthesis of non-coding Pol II transcripts, including enhancer RNAs (eRNAs), snRNAs, telomerase RNAs, and lncRNAs. Related references: PMID:32647223

• Within the integrator tail module, INTS13 serves as a recruitment platform for transcription factors at promoters. Related references: PMID:38823386, PMID:38906142

• INTS13 localizes to both the nucleus and cytoplasm. At prophase, it mediates cytoplasmic dynein recruitment to the nuclear envelope, facilitating centrosome–nucleus coupling. During G2/M, it supports proper spindle formation and cytokinesis. Related references: PMID:23097494, PMID:23904267

• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin-like conjugation. The protein is widely expressed, with a tendency for upregulation in seminomas compared to normal testis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 200–300 of human INTS13; consider verifying epitope accessibility in native protein contexts.
• For western blotting, expect a band near 80 kDa. Validate with a positive control such as HeLa or HEK293 cell lysates and confirm specificity using siRNA knockdown or knockout samples.
• For ELISA, titrate the antibody against recombinant antigen or cell lysates to determine optimal working concentration.
• Reported cross-reactivity is for human; if analysis of other species is required, perform sequence alignment and experimental verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing bridge for high-quality IVD raw materials. This anti-INTS13 monoclonal antibody supports the development of assays targeting transcription termination and cell cycle regulation. Beyond individual antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and access to rare-target raw materials—streamlining procurement from concept to clinic.