Anti-ASS1 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P00966

Core Product Specifications and Parameters

Biological Background: ASS1 Function and Localization

• ASS1 encodes argininosuccinate synthase, also known as citrulline--aspartate ligase (UniProt P00966).
• Catalyzes the ATP-dependent ligation of citrulline and aspartate to form argininosuccinate, a critical step in the urea cycle for ammonia detoxification and arginine biosynthesis.
• Functions in the cytosol and is highly expressed in the adult liver, the primary site of ureagenesis.
• Requires ATP and nucleotide binding for its ligase activity.
• Subject to post-translational modifications including acetylation and phosphorylation, which may regulate its function.
• Mutations in ASS1 are associated with citrullinemia type I, an autosomal recessive urea cycle disorder.
• Structural studies provide detailed insight into the catalytic mechanism of this enzyme.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human ASS1; thus the antibody is predicted to bind full-length and N-terminal fragments.
• For Western blot, a band around 47 kDa is expected; appropriate loading controls and molecular weight markers are recommended.
• In immunofluorescence, cytoplasmic staining should be observed; consider using liver tissue as a positive control, given high expression in hepatocytes.
• Cross-reactivity with mouse and rat ASS1 is reported; validate in the relevant sample system before large-scale studies.
• For ELISA, test with recombinant ASS1 protein or cell lysates; optimal antibody concentration should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research teams comprehensive access to IVD raw materials and technical services, from early R&D to clinical application. This anti-ASS1 rabbit monoclonal antibody supports urea cycle disorder and arginine metabolism research, and we can supply matched antibody pairs, bulk monoclonal/polyclonal reagents, and ancillary consumables to streamline your assay development. Explore our full portfolio for rare-target sourcing and custom solutions.