Anti-ASPSCR1 (TUG) Rabbit Polyclonal Antibody for WB, IF/ICC, IP - Q9BZE9

Core Product Specifications and Parameters

Biological Background: TUG/ASPSCR1 Function and Localization

• ASPSCR1 (also known as TUG, UBXD9) encodes a tethering protein that retains GLUT4-containing vesicles in the cytoplasm under basal conditions, thereby regulating insulin-stimulated glucose uptake.
• It modulates the cell-surface availability of the GLUT4 glucose transporter by controlling vesicle sequestration, a key process in insulin-responsive glucose homeostasis.
• ASPSCR1 enhances VCP/p97 methylation catalyzed by VCPKMT, suggesting an involvement in ubiquitin-dependent protein quality control pathways.
• The protein localizes predominantly to the endomembrane system, particularly the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membrane, and is also found in the cytoplasm and nucleus.
• Expression is ubiquitous, with highest levels observed in testis, heart, skeletal muscle, and pancreas — tissues integral to energy metabolism and insulin action.
• Post-translational modifications include phosphorylation and acetylation, and the gene undergoes alternative splicing, yielding multiple isoforms.
• Chromosomal rearrangements involving ASPSCR1 are implicated in alveolar soft part sarcoma (ASPS) and renal cell carcinoma, classifying it as a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 284-553, a region that may include the UBX domain, supporting detection of full-length ASPSCR1. For Western blot, expect a band near the predicted 60 kDa molecular weight.
• For immunofluorescence, consider that ASPSCR1 localizes to the ERGIC and cytoplasm with possible nuclear staining; validate in your cell type using appropriate organelle markers.
• This polyclonal antibody may be suitable for immunoprecipitation of endogenous ASPSCR1 complexes; lysis buffer conditions and antibody-to-lysate ratios should be optimized.
• In ELISA applications, the antibody can be evaluated for both capture and detection roles, but assay parameters must be empirically determined.
• Always include appropriate negative controls (e.g., siRNA knockdown or knockout lysates) to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, supplying validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents for assay development. This ASPSCR1 polyclonal antibody supports research into GLUT4 trafficking, insulin signaling, and cancer biology. Beyond standard catalog items, we can assist with custom antibody generation and rare target sourcing to accelerate your diagnostic projects.