Anti-ASPH Polyclonal Antibody for IF/ICC, ELISA - Q12797

Core Product Specifications and Parameters

Biological Background: ASPH Function and Localization

• ASPH encodes aspartyl/asparaginyl beta-hydroxylase, which specifically hydroxylates Asp or Asn residues in certain epidermal growth factor (EGF)-like domains of target proteins.
• The protein also functions as a membrane-bound Ca(2+)-sensing protein and is a structural component of endoplasmic reticulum-plasma membrane junctions. Isoform 8 regulates calcium release-activated calcium (CRAC) channels in T-cells.
• Alternative splicing generates multiple isoforms; isoform 1 is broadly expressed, while isoform 8 is mainly expressed in pancreas, heart, brain, kidney, and liver.
• ASPH localizes to the endoplasmic reticulum membrane and sarcoplasmic reticulum membrane, consistent with its roles in protein modification and calcium signaling.
• The protein belongs to the iron/2-oxoglutarate-dependent dioxygenase family, requiring iron as a cofactor, and contains TPR repeat domains.
• Keywords such as glycoprotein, phosphoprotein, disulfide bond, and transmembrane helix indicate post-translational modifications and membrane integration.
• A disease variant association suggests potential roles in pathology, but specific functional implications require further investigation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 81-270 of human ASPH, covering part of the catalytic domain; this region is expected to generate antibodies recognizing full-length and potentially isoforms containing this sequence.
• For IF/ICC, consider optimizing fixation and permeabilization conditions due to the membrane localization of ASPH.
• For ELISA, the antibody may be used for antigen detection or capture; validate with appropriate positive and negative controls.
• Since ASPH is expressed in multiple tissues, include tissue-specific lysates or cell lines to confirm specificity.

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CamelBio supports diagnostic developers and researchers with reliable access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-ASPH product. Whether you require bulk ancillary reagents or sourcing for rare targets in post-translational modification and calcium signaling research, our one-stop platform streamlines procurement from concept to clinic.