Anti-ASPA Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P45381

Core Product Specifications and Parameters

Biological Background: ASPA Function and Localization

• Aspartoacylase (ASPA), also known as aminoacylase-2 (ACY-2), is a zinc-dependent hydrolase that catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate.
• NAA is present in high concentrations in the brain, and its hydrolysis by ASPA is critical for maintaining intact white matter integrity.
• In peripheral tissues, ASPA functions as a scavenger of NAA from body fluids, contributing to systemic metabolite homeostasis.
• ASPA localizes to both the cytoplasm and nucleus, indicating potential roles in nuclear metabolic processes.
• Tissue expression of ASPA is predominantly in brain white matter, skeletal muscle, kidney, adrenal glands, lung, and liver.
• Mutations in the ASPA gene are associated with Canavan disease, a leukodystrophy characterized by spongy degeneration of the brain.
• ASPA has been resolved by 3D-structure analysis, providing insights into its zinc-binding catalytic mechanism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human ASPA protein (amino acids 1–313), so the antibody may recognize native and denatured ASPA.
• For Western blotting, the predicted molecular weight is ~36 kDa; consider that post-translational modifications could cause minor shifts.
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions as ASPA is an intracellular protein localized to cytoplasm and nucleus.
• The antibody shows cross-reactivity with mouse and rat ASPA; validate in the appropriate tissue or cell line for your experimental system.
• For ELISA applications, pairing with a detection antibody may require testing due to polyclonal nature.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic and research communities by offering a comprehensive portfolio of IVD raw materials, including this anti-ASPA polyclonal antibody. Our one-stop sourcing bridge ensures access to validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for neurology and leukodystrophy research. Whether you need established or hard-to-find targets, CamelBio streamlines procurement from concept to clinic.