Anti-ASNS Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P08243

Core Product Specifications and Parameters

Biological Background: ASNS Function and Localization

• Asparagine synthetase (ASNS) catalyzes the glutamine- and ATP-dependent conversion of aspartic acid to asparagine, a critical step in amino acid biosynthesis.
• Functions as a ligase with glutamine amidotransferase activity, using ATP as a nucleotide-binding cofactor.
• Plays a role in cell cycle control; also known as cell cycle control protein TS11.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its activity.
• Alternative splicing generates multiple isoforms with potentially distinct functions.
• Mutations in ASNS are associated with asparagine synthetase deficiency, a neurodevelopmental disorder characterized by intellectual disability (disease variant).
• The protein has a molecular weight of approximately 64 kDa and is identified in proteomics screens (Reference proteome).
• A 3D-structure has been determined, providing insights into its catalytic mechanism.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of ASNS is ~64 kDa; validate detection using positive control lysates from cell lines known to express ASNS (e.g., human, mouse, or rat tissues).
• In immunofluorescence/immunocytochemistry, consider optimizing fixation and permeabilization conditions to accurately visualize endogenous ASNS localization.
• For ELISA applications, the antibody can be used as a capture or detection reagent; pair with an appropriately matched antibody and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, offering this anti-ASNS antibody to support research in amino acid metabolism and neurodevelopment. Beyond single antibodies, we supply validated antibody pairs, bulk reagents, and custom sourcing for rare targets, ensuring seamless transition from concept to clinical application.