Anti-ASNA1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O43681

Core Product Specifications and Parameters

Biological Background: ASNA1 (GET3) Function and Localization

• ASNA1, also known as ATPase GET3, ARSA-I, or TRC40, is an ATPase that facilitates the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum membrane.
  Related references: PMID:17382883
• The enzyme recognizes the transmembrane domain of newly synthesized TA proteins in the cytosol and forms a targeting complex that interacts with the ER membrane receptors GET1/WRB and CAMLG/GET2.
• ATP binding and hydrolysis control conformational transitions: ATP binding drives homodimer closure for substrate recognition, while hydrolysis is required for TA protein insertion and subsequent recycling of GET3.
• ASNA1 may also participate in insulin signaling.
• Subcellularly, GET3 localizes to the cytoplasm, endoplasmic reticulum, and nucleus (including the nucleolus), reflecting its dynamic targeting cycle.
• Tissue expression analysis shows ASNA1 present in epithelial cells of the liver, kidney, and stomach, as well as in adrenal medulla, pancreatic islets, spleen red pulp, and cardiac/skeletal muscle.
• ASNA1 is classified as a hydrolase, an ATP-binding protein, and a zinc metalloprotein, and is linked to cardiomyopathy and other disease variants.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering amino acids 1-348 of human ASNA1, which represents the full-length protein. This suggests the antibody may recognize multiple epitopes across the entire protein.
• For Western blot (WB), expect a band at approximately 39 kDa (theoretical molecular weight ~38.8 kDa) in whole cell lysates from human, mouse, or rat tissues.
• When performing immunofluorescence (IF/ICC), consider permeabilization protocols that allow antibody access to cytoplasmic, ER, and nuclear compartments, as ASNA1 localizes to all three.
• Validate antibody specificity in your experimental system using appropriate controls (e.g., knockdown, blocking peptide) to confirm signal authenticity, especially given potential nucleolar staining.
• For ELISA, the antibody can be used as a capture or detection reagent, but optimal dilution and pair compatibility should be determined experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined pathway to high-quality IVD raw materials and technical support—from concept to clinic. This ASNA1 (GET3) antibody supports investigations into tail-anchored protein trafficking, ER biology, and related disease mechanisms. Through our extensive sourcing network, we deliver validated antibody pairs, optimized monoclonals/polyclonals, ancillary reagents, and can source rare-target raw materials to accelerate your assay development.