Anti-ASMT Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P46597

Core Product Specifications and Parameters

Biological Background: ASMT Function and Localization

• ASMT (Acetylserotonin O-methyltransferase), also known as Hydroxyindole O-methyltransferase (HIOMT), is the key enzyme that catalyzes the final step in melatonin biosynthesis: the transfer of a methyl group from S-adenosyl-L-methionine onto N-acetylserotonin to produce melatonin (N-acetyl-5-methoxytryptamine).
• The ASMT gene undergoes alternative splicing, resulting in multiple transcript variants that encode different protein isoforms.
• At the protein level, ASMT is predominantly expressed in the pineal gland, where it plays a critical role in regulating circadian rhythms and seasonal reproduction via melatonin secretion.
• In the retina, very low mRNA expression has been detected, but ASMT protein is absent, indicating tissue-specific regulation of this enzyme. Related references: PMID:7989373 PMID:8574683
• ASMT belongs to the methyltransferase superfamily and requires S-adenosyl-L-methionine as a methyl donor for its catalytic activity.
• The protein is annotated under lipid metabolism, highlighting a potential link between melatonin synthesis and broader metabolic pathways.
• Structural studies (evidenced by 3D-structure annotations) have revealed the enzyme's active site architecture, facilitating research into its catalytic mechanism and inhibitor design.
• Mass spectrometry-based proteomics has confirmed ASMT expression in the pineal gland, supporting its utility as a biomarker for pineal function.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-298 of human ASMT (NP_001164510.1). This nearly full-length sequence is likely to generate antibodies recognizing multiple linear epitopes, making it suitable for detecting denatured protein in Western blot.
• For IHC-P, consider optimizing antigen retrieval conditions and antibody dilution, as polyclonal antibodies may exhibit higher background in formaldehyde-fixed, paraffin-embedded tissues.
• In ELISA, the antibody can detect recombinant or native ASMT; however, cross-reactivity with other methyltransferases should be validated, especially when testing samples from species with high sequence homology.
• When using this antibody in mouse or rat tissues, confirm cross-reactivity through sequence alignment or preliminary titration, given that the immunogen is human-derived.
• For WB, expect a band at approximately 38 kDa; additional bands from isoforms or post-translational modifications may appear and should be verified with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to streamline IVD raw-material procurement, from lead identification to clinical translation. For melatonin biosynthesis and circadian rhythm research, we provide validated antibody pairs, recombinant proteins, and ancillary reagents to accelerate assay development. Contact our team for bulk pricing, custom formulations, and rare-target raw-material sourcing support.