Anti-ASL Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04424

Core Product Specifications and Parameters

Biological Background: ASL Function and Localization

• ASL encodes argininosuccinate lyase, a homotetrameric enzyme of the urea cycle that catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, a critical step for hepatic nitrogen detoxification and de novo arginine synthesis in nonhepatic tissues. Related references: PMID:11747432 PMID:11747433 PMID:22081021
• ASL is an essential regulator of intracellular and extracellular L-arginine pools.
• In the citrulline-nitric oxide cycle, ASL assembles into tissue-specific multiprotein complexes with argininosuccinate synthase (ASS1), the cationic amino acid transporter SLC7A1, and nitric oxide synthase isoforms (NOS1, NOS2, or NOS3) to enable cell-autonomous L-arginine synthesis and channel extracellular L-arginine into nitric oxide production. Related references: PMID:22081021
• Alternative splicing generates multiple ASL transcript variants, and the protein undergoes post-translational modifications such as acetylation.
• The protein has a predicted molecular weight of approximately 52 kDa (UniProt: 51658 Da).

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected band for endogenous ASL is around 52 kDa; consider optimizing primary antibody dilution and using appropriate loading controls.
• For immunohistochemistry paraffin-embedded tissues (IHC-P), heat-induced antigen retrieval with citrate buffer (pH 6.0) is recommended to unmask epitopes.
• In immunofluorescence/immunocytochemistry (IF/ICC), fix cells with 4% paraformaldehyde and permeabilize with 0.1% Triton X-100 before staining; counterstaining with nuclear dyes may help localize the cytosolic enzyme.
• As a polyclonal antibody, it may recognize multiple epitopes and potential isoforms; validate specificity in the relevant species and tissue system.
• For ELISA, the antibody can be used for antigen capture or detection; appropriate coating and blocking conditions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, providing high-quality antibodies, antigens, and ancillary reagents for diagnostic and research applications. This anti-ASL polyclonal antibody supports studies of urea cycle function and arginine metabolism, and we offer bulk ordering, custom development, and technical consultation to streamline your project from concept to clinic. Whether you need validated antibody pairs, optimized monoclonal alternatives, or rare-target sourcing, CamelBio is your procurement bridge for reliable, scalable solutions.