Anti-ASH2L Monoclonal Antibody for WB, IHC, IF, ELISA - Q9UBL3

Core Product Specifications and Parameters

Biological Background: ASH2L Function and Localization

• ASH2L (ASH2-like protein, also known as ASH2, Bre2, ASH2L1, ASH2L2) is a core component of the Set1/Ash2 histone methyltransferase (HMT) complex, which specifically methylates lysine 4 of histone H3 (H3K4), a mark associated with active transcription. Related references: PMID:12670868 PMID:19131338 PMID:19556245

• It acts as a transcriptional regulator by recruiting histone methyltransferase complexes to gene promoters, thereby modulating chromatin structure and gene expression. Related references: PMID:19131338

• Within the MLL1/MLL complex, ASH2L is crucial for mono- and dimethylation of H3K4, and it synergistically stimulates the methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A, and SETD1B when associated with RBBP5 and WDR5. Related references: PMID:19556245 PMID:21220120 PMID:22266653

• ASH2L may also play a role in hematopoiesis, as suggested by its expression pattern and functional studies. Related references: PMID:12670868

• The protein is localized predominantly in the nucleus, consistent with its function in chromatin regulation.

• It is ubiquitously expressed, with higher levels in adult heart and testis and in fetal lung and liver, and barely detectable in adult lung, liver, kidney, prostate, and peripheral leukocytes.

• ASH2L undergoes post-translational modifications including acetylation, phosphorylation, and methylation, and contains zinc-finger domains, indicating a capacity for DNA binding and metal coordination.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 529–628 of human ASH2L (Q9UBL3), which lies within the C-terminal region. When used in Western blotting (WB), anticipate a major band near the predicted molecular weight of 69 kDa; additional bands may reflect alternative splicing or post-translational modifications.
• For immunofluorescence (IF/ICC and IF-P) and immunohistochemistry (IHC-P), consider performing antigen retrieval and optimizing fixation conditions, as nuclear epitopes can vary in accessibility.
• Given the antibody's cross-reactivity with mouse and rat ASH2L, it is suitable for comparative studies in rodent cell lines or tissues; however, validation in the relevant sample system is recommended.
• While the antibody is listed for ELISA, users should titrate and validate under their specific assay conditions to confirm sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop sourcing partner for IVD raw materials, offering diagnostic manufacturers and research laboratories convenient access to validated antibody products like this anti-ASH2L monoclonal antibody. Our catalog includes optimized antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing services, supporting your projects in epigenetics, chromatin biology, and transcription regulation from early development through to clinical application.