Anti-ASGR2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P07307

Core Product Specifications and Parameters

Biological Background: ASGR2 Function and Localization

• ASGR2 encodes the asialoglycoprotein receptor 2, a transmembrane C-type lectin that recognizes and binds terminal galactose and N-acetylgalactosamine residues on desialylated glycoproteins.
• It functions as a recycling endocytic receptor: upon ligand binding, the receptor-ligand complex is internalized, transported to sorting endosomes, dissociated, and the receptor recycles back to the plasma membrane.
• ASGR2 is a member of the C-type lectin domain family (CLEC4H2) and is highly expressed exclusively in hepatic parenchymal cells, underscoring its tissue-specific role in liver clearance of desialylated serum glycoproteins and lipoproteins.
• The receptor contains key structural features including a calcium-dependent carbohydrate recognition domain, disulfide bonds, a transmembrane helix, and a signal-anchor sequence, and undergoes post-translational modifications such as palmitoylation and phosphorylation.
• ASGR2 exists in alternatively spliced isoforms and has been implicated in host-virus interactions, serving as an entry receptor for certain viral pathogens.
• The full-length protein has a predicted molecular weight of ~35 kDa (35,092 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 58-287 of human ASGR2 (NP_550435.1), covering the extracellular lectin domain, which may contribute to recognition of the native receptor.
• For Western blotting (WB), the predicted molecular weight is ~35 kDa; consider including appropriate controls and validating antibody specificity in liver tissue or hepatocyte-derived cell lines.
• In immunofluorescence (IF-P), it is advisable to optimize permeabilization and fixation conditions to detect membrane-localized ASGR2 in hepatic cells.
• ELISA applications may require titration of the antibody to achieve optimal signal-to-noise ratio; recombinant ASGR2 protein can serve as a positive control.
• Cross-reactivity with mouse ASGR2 is reported; researchers should verify reactivity in their experimental models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined procurement channel for high-quality IVD raw materials. For liver function and endocytosis studies, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like ASGR2. Partner with CamelBio to bridge your development from concept to clinic.