Anti-ASGR1 Polyclonal Antibody for WB, IHC-P, ELISA - P07306

Core Product Specifications and Parameters

Biological Background: ASGR1 Function and Localization

• ASGR1 encodes the asialoglycoprotein receptor 1 (ASGPR1), also known as C-type lectin domain family 4 member H1 (CLEC4H1) or hepatic lectin H1 (HL-1), a transmembrane protein predominantly expressed on hepatocytes.
• Mediates the endocytosis of desialylated plasma glycoproteins, contributing to the clearance of aged platelets and activated lymphocytes, and maintaining serum glycoprotein homeostasis. Related references: PMID:25485912
• Functions in platelet homeostasis by activating a JAK2-STAT3 feedback loop that regulates thrombopoietin (TPO) expression upon binding desialylated platelets.
• Participates in immune surveillance by recognizing and eliminating activated lymphocytes; hepatocytes act as cytotoxic effectors during this process. Related references: PMID:21656538
• May also play a role in lipid homeostasis (by similarity).
• Acts as an alternate receptor for ACE2-independent SARS-CoV-2 entry, highlighting its role in host-virus interactions.
• Subcellular localization: primarily found on the cell membrane; also detected as a secreted form.
• Tissue specificity: expression is restricted to hepatic parenchymal cells, making it a liver-specific marker.
• Key structural features include a C-type lectin domain, calcium-dependent activity, a transmembrane helix, and post-translational modifications such as palmitoylation, phosphorylation, and disulfide bond formation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–150 of human ASGR1 (NP_001662.1), covering the N-terminal extracellular region, which may enable detection of both membrane-bound and secreted forms.
• For Western blotting (WB), the predicted molecular weight is approximately 33 kDa; however, glycosylation may result in a higher observed band. Validate in the relevant sample system.
• For immunohistochemistry (IHC-P), strong signal is expected in liver tissue sections. Include appropriate positive (liver) and negative (non-hepatic) tissue controls.
• For ELISA, consider pairing this antibody with another clone or a monoclonal antibody for sandwich assay development; perform cross-reactivity testing.
• Cross-reactivity with mouse and rat has been confirmed; for other species, validation is recommended before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio equips diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This high-quality Anti-ASGR1 polyclonal antibody is an excellent choice for studying liver function, glycoprotein clearance pathways, and viral entry mechanisms. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal formulations, bulk ancillary reagents, and rare target raw-material sourcing capabilities—empowering your diagnostic and research workflows with reliable and scalable solutions.