Anti-ASF1B Rabbit Polyclonal Antibody for WB, ELISA - Q9NVP2

Core Product Specifications and Parameters

Biological Background: Histone chaperone ASF1B Function and Localization

• ASF1B (Anti-silencing function protein 1 homolog B), also known as CIA‑II or hAsf1b, is a histone chaperone encoded by the ASF1B gene in humans.
• It facilitates histone deposition and exchange during nucleosome assembly and disassembly, cooperating with chromatin assembly factor 1 (CAF‑1) to promote replication‑dependent chromatin assembly. Related references: PMID:11897662, PMID:14718166, PMID:15664198
• ASF1B specifically binds newly synthesized histones H3‑H4 with monomethylated H3K9 (H3K9me1) and diacetylated H4K5K12 (H4K5K12ac) marks, mediating their nuclear import together with importin‑4 (IPO4). Related references: PMID:20953179, PMID:21454524, PMID:26527279
• Unlike its paralog ASF1A, ASF1B does not participate in replication‑independent nucleosome deposition mediated by the HIRA complex. Related references: PMID:11897662, PMID:14718166, PMID:15664198
• Subcellularly, ASF1B is found in the nucleus, cytoplasm, and cytosol, shuttling between compartments to escort histones.
• It is required for gonad development and is highly expressed in testis, with lower expression in colon, small intestine, and thymus. Related references: PMID:12842904
• ASF1B is a phosphoprotein and is implicated in developmental processes, differentiation, spermatogenesis, and transcription regulation.

Experimental Guidance and Technical Tips

• As a rabbit polyclonal antibody, this product may recognize multiple epitopes; consider validating specificity in your target sample system.
• For Western blot, the antibody is expected to detect endogenous ASF1B at approximately 22 kDa in human cell or tissue lysates.
• In ELISA, the immunogen (recombinant ASF1B fragment) can serve as a positive control; optimize coating concentration and detection conditions.
• Cross‑reactivity with species other than human is not guaranteed; if needed, test the antibody in the relevant model organism.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials for chromatin biology and epigenetics research, including antibodies targeting histone chaperones such as ASF1B. As your one‑stop sourcing partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist with rare‑target raw‑material procurement—helping diagnostic manufacturers streamline development from concept to clinic.