Anti-ASC/TMS1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q9ULZ3

Core Product Specifications and Parameters

Biological Background: ASC/TMS1 Function and Localization

• Apoptosis-associated speck-like protein containing a CARD (ASC/TMS1) is a key mediator of apoptosis and inflammation. It promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Related references: PMID:11103777, PMID:12646168
• ASC functions as an integral adapter in the assembly of various inflammasomes (NLRP1, NLRP2, NLRP3, NLRP6, AIM2 and probably IFI16), which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines. Related references: PMID:15030775, PMID:16982856, PMID:17349957
• Through its pyrin and CARD domains, ASC mediates homotypic interactions that nucleate the formation of caspase-1 filaments, acting as a platform for caspase-1 polymerization and macrophage pyroptosis. Related references: PMID:14499617, PMID:19234215, PMID:24630722
• ASC is localized to the cytoplasm, nucleus, endoplasmic reticulum, mitochondria, and Golgi apparatus membrane; it is recruited to inflammasomes upon activation.
• It also modulates transcriptional activation of cytokines and chemokines independent of the inflammasome, involving AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. Related references: PMID:12486103, PMID:16585594
• Widely expressed at low levels, detected in peripheral blood leukocytes, lung, small intestine, spleen, and colon; expression confirmed in lung epithelial cells at protein level. Related references: PMID:23229815
• Post-translational modifications include phosphorylation and Ubl conjugation (isopeptide bond); the protein is implicated in tumor suppression.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 91 amino acids, which includes the pyrin domain (PYD); the antibody may recognize both full-length and any N-terminal isoforms. For applications requiring detection of specific isoforms, validation with appropriate controls is recommended.
• For Western blot (WB), a primary band around 22 kDa is expected; however, potential post-translational modifications or cleavage fragments may produce additional bands; loading controls and molecular weight markers should be used.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), permeabilization and fixation conditions should be optimized, as ASC localizes to multiple subcellular compartments. Nuclear and cytoplasmic staining patterns may vary depending on activation state.
• Immunoprecipitation (IP) and ELISA applications are also validated; consider using fresh lysates and including protease inhibitors to preserve protein interactions.
• Cross-reactivity with mouse and rat is reported; however, sequence homology should be checked for studies in these species, and pilot experiments are advised.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing platform, CamelBio supports diagnostic developers with validated antibodies, bulk reagents, and rare target sourcing. This anti-ASC/TMS1 antibody caters to research in apoptosis, inflammasome biology, and immune regulation. Explore our portfolio for matched pairs, ancillary reagents, and custom sourcing solutions to accelerate your assay development from concept to clinic.