Anti-ASCT2/SLC1A5 Monoclonal Antibody for WB, IHC-P, ELISA - Q15758

Core Product Specifications and Parameters

Biological Background: ASCT2/SLC1A5 Function and Localization

• Neutral amino acid transporter B(0) (gene SLC1A5), commonly referred to as ASCT2, RDR, RDRC, M7V1, or ATBO, is a multi-pass transmembrane protein belonging to the solute carrier family 1.
  Related references: PMID:17094966, PMID:23756778, PMID:24953180, PMID:26492990
• Functions as a sodium-coupled antiporter that preferentially exchanges extracellular L-glutamine with intracellular neutral amino acids (e.g., L-serine, L-threonine, L-asparagine), fueling biosynthetic pathways and energy metabolism in proliferating cells.
  Related references: PMID:17094966, PMID:38650461
• Mediates the transport of D-serine in the retina and renal proximal tubules, thereby influencing NMDA receptor modulation and systemic D-serine homeostasis.
  Related references: PMID:10708449, PMID:23492904, PMID:38671230
• Serves as a cell surface receptor for several retroviruses, including RD114, baboon M7, and type D simian retroviruses, and plays a role in syncytin-1-dependent trophoblast fusion during placental development.
• Localizes predominantly to the cell membrane and apical cell membrane; also detected in melanosomes.
  Related references: PMID:8702519
• Expressed in placenta, lung, skeletal muscle, kidney, pancreas, and intestine, with prominent expression in CD34-positive hematopoietic progenitors.
• Undergoes acetylation and phosphorylation; annotated as a glycoprotein, phosphoprotein, and metal-binding membrane receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal intracellular domain (amino acids 483–541), suggesting the antibody should recognize the full‑length protein; confirm specificity in a relevant SLC1A5‑expressing cell line.
• For Western blot, the predicted molecular weight is approximately 57 kDa; include a positive control (e.g., human placenta or cancer cell lysate) and use standard reducing conditions.
• In immunohistochemistry (IHC-P), optimize antigen retrieval (citrate or EDTA buffer) and validate staining using tissue sections known to express ASCT2 (e.g., kidney, intestine).
• For ELISA assays, this monoclonal antibody can be explored as a capture or detection reagent; pair with a matched detection antibody and verify performance in the intended sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner offering a wide range of validated antibodies, recombinant proteins, and ancillary reagents to accelerate diagnostic development from concept to clinic. This anti‑ASCT2/SLC1A5 antibody supports research in amino acid transport, cancer metabolism, and viral entry pathways. For bulk orders, custom antibody pairs, or sourcing rare‑target raw materials, contact us to design a supply solution tailored to your assay requirements.