Anti-ASCL4 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q6XD76

Core Product Specifications and Parameters

Biological Background: ASCL4 Function and Localization

• ASCL4 (Achaete-scute homolog 4), also known as ASH-4, HASH4, or bHLHa44, is a member of the basic helix-loop-helix (bHLH) family of transcription factors.
• It is predicted to function as a transcriptional regulator, with a potential role in skin development.
• The ASCL4 gene encodes a 19 kDa protein (calculated molecular weight 19253 Da) localized primarily in the nucleus.
• Subcellular localization is exclusively nuclear, consistent with its role as a DNA-binding transcription factor.
• Tissue expression is predominantly in skin, with a 7-fold higher level in fetal skin compared to adult skin, suggesting a developmental role.
• Weak expression is also detected in fetal lung, aorta, and brain, as well as in adult stomach, kidney, ovary, and breast.
• Keywords associated with ASCL4 include DNA-binding, Nucleus, Transcription, and Transcription regulation, underscoring its function in gene expression control.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the N-terminal region (amino acids 1-70) of human ASCL4. Consider verifying the antibody's specificity using appropriate positive controls (e.g., skin tissue lysates) in your assay.
• For western blot, use skin-derived cell lines or tissue homogenates where ASCL4 is expressed. The predicted molecular weight is ~19 kDa; validate the observed band against this size.
• In IHC-P, skin sections (fetal or adult) are recommended as positive controls. Optimize antigen retrieval and antibody dilution for your specific sample type.
• Cross-reactivity with mouse and rat has been reported; however, confirm sequence homology in the immunogen region before use in these species.
• Perform appropriate negative controls (e.g., blocking peptide or knockout cell lines) to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and clinical institutions with a comprehensive range of IVD raw materials and technical support. The ASCL4 antibody product supports studies in transcriptional regulation and skin development, aiding the development of relevant diagnostic assays. In addition, we offer validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, ensuring a seamless transition from reagent selection to clinical application.