Anti-ASCL4 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q6XD76

Core Product Specifications and Parameters

Biological Background: ASCL4 Function and Localization

• ASCL4 (Achaete-scute homolog 4), also known as ASH-4, HASH4, or bHLHa44, is a member of the basic helix-loop-helix (bHLH) family of transcription factors.
• It is predicted to function as a transcriptional regulator, with a suggested role in skin development based on expression patterns.
• ASCL4 is predominantly expressed in the skin, with markedly higher levels in fetal skin compared to adult skin.
• Lower expression is also detected in fetal lung, aorta, and brain, as well as adult stomach, kidney, ovary, and breast.
• The protein localizes to the nucleus, consistent with its role as a DNA-binding transcription factor.
• UniProt annotations highlight its involvement in transcription regulation and DNA binding (Keywords: DNA-binding, Nucleus, Transcription, Transcription regulation).
• The predicted molecular weight is approximately 19.3 kDa (19253 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100) of human ASCL4, which may contribute to specificity.
• Western blot (WB): Consider testing at a range of dilutions on lysates from skin or other expressing tissues (e.g., A431, HaCaT) to detect the predicted ~19 kDa band. Validate with positive and negative controls.
• Immunofluorescence/immunocytochemistry (IF/ICC): Nuclear staining is expected; permeabilization and appropriate fixation may be critical. Use relevant cell lines such as keratinocytes.
• ELISA: Suitable for detecting ASCL4 in samples; optimize coating and detection conditions based on the assay format.
• Cross-reactivity is reported for human; for other species, validate experimentally if needed.

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CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated monoclonal antibodies like this anti-ASCL4 rabbit mAb for research in dermatology and developmental biology. We provide optimized antibody pairs, bulk reagents, and rare target sourcing to support your assay development from concept to clinic.