Anti-ASCC3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8N3C0

Core Product Specifications and Parameters

Biological Background: ASCC3 Function and Localization

• ASCC3, also known as ASC1p200 or HELIC1, is a ~251 kDa protein encoded by the ASCC3 gene in humans, belonging to the helicase superfamily with ATP-binding and hydrolase activities.
• ASCC3 functions as a 3'-5' DNA helicase, unwinding DNA to provide single-stranded substrate for ALKBH3, enabling repair of alkylated N3-methylcytosine lesions. Related references: PMID:22055184
• As part of the ribosome quality control trigger (RQT) complex, ASCC3 drives splitting of stalled, ubiquitinated ribosomes to rescue translational issues, ensuring nascent peptide degradation. Related references: PMID:28757607, PMID:32099016, PMID:32579943
• ASCC3 is a subunit of the ASC-1 complex, which acts as a transcriptional coactivator enhancing NF-kappa-B, SRF, and AP1 transactivation. Related references: PMID:12077347
• Subcellularly, ASCC3 localizes primarily to the nucleus and nuclear speckles, but is also found in the cytoplasm and cytosol.
• The protein is subject to post-translational modifications including acetylation and phosphorylation, and undergoes alternative splicing, resulting in multiple isoforms.
• ASCC3 is ubiquitously expressed across tissues, consistent with its roles in fundamental processes like DNA repair and translation quality control.
• Mutations in ASCC3 are associated with intellectual disability, highlighting its critical functions in cellular homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal amino acids 1-111, so the antibody is expected to recognize the N-terminus of human ASCC3.
• For Western blot, a major band at ~251 kDa is anticipated; consider using specialized large-protein transfer conditions (e.g., low methanol, extended transfer time) to ensure efficient detection.
• For IF/ICC, validate the reported nuclear/cytoplasmic localization using appropriate fixation and permeabilization methods, and include controls for species cross-reactivity when analyzing mouse cells or tissues.
• As a polyclonal antibody, batch-to-batch consistency may vary; it is advisable to perform pre-adsorption tests if high specificity against particular isoforms or modifications is required.
• ELISA applications may require optimizing coating antigen and antibody dilutions based on the specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This ASCC3 polyclonal antibody supports research in DNA damage repair and translational quality control, areas critical for cancer and neurodegenerative disease biomarker development. Beyond individual antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and rare target raw-material sourcing to streamline your IVD assay development.