Anti-ASCC1 Rabbit Polyclonal Antibody for WB, ELISA - Q8N9N2

Core Product Specifications and Parameters

Biological Background: ASCC1 Function and Localization

• ASCC1 (Activating signal cointegrator 1 complex subunit 1), also known as ASC-1 complex subunit p50 or Trip4 complex subunit p50, is encoded by the ASCC1 gene (GeneID 51008) in humans. The protein has a calculated molecular weight of approximately 46 kDa (UniProt: 45.5 kDa).
• ASCC1 is a key component of the ASC-1 (Activating signal cointegrator 1) complex, which enhances the transactivation of transcription factors NF-kappa-B, SRF, and AP1. Related references: PMID:12077347
• As part of the ASCC complex, ASCC1 plays a critical role in DNA damage repair, facilitating the resolution of alkylation damage. Related references: PMID:29997253
• In response to gastrin-activated paracrine signals, ASCC1 is involved in the induction of SERPINB2 expression, highlighting a role in cellular signaling and gene regulation.
• The protein is localized in the nucleus, specifically within nuclear speckles, consistent with its roles in transcription and DNA repair.
• ASCC1 is ubiquitously expressed across human tissues, supporting its fundamental cellular functions.
• Additional post-translational modifications, alternative splicing, and direct protein sequencing have been reported; the protein is implicated in neurodegenerative processes as well (UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-180) of human ASCC1, which may help detect full-length and possibly some truncated isoforms; consider verifying isoform specificity in your sample system.
• For WB, a band at approximately 46 kDa is expected in human, mouse, and rat cell lysates; optimize blocking and antibody dilution to minimize background.
• ELISA can be used for quantitative detection; validate the assay with recombinant ASCC1 protein or known positive controls.
• Cross-reactivity with mouse and rat is indicated, but always confirm reactivity in your experimental model.

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