Anti-ASB3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y575

Core Product Specifications and Parameters

Biological Background: ASB3 Function and Localization

• ASB3 (Ankyrin repeat and SOCS box protein 3) is the probable substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins.
• It recognizes TNFRSF1B, promoting its ubiquitin-dependent degradation. Related references: PMID:15899873
• Plays a role in down-regulation of antiviral innate immunity by targeting MAVS for ubiquitin-proteasomal degradation. Related references: PMID:39266719
• Destabilizes TRAF6 by enhancing its 'Lys-48'-linked polyubiquitination. Related references: PMID:39162488
• Subcellular localization is predominantly cytoplasmic.
• Contains characteristic ANK repeats and a SOCS box domain; involved in the ubiquitin conjugation pathway.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-145 of human ASB3, targeting the N-terminal region. This region likely yields broad isoform recognition.
• For Western blotting, a band around 58 kDa is expected; validate the observed molecular weight in your specific sample system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), appropriate antigen retrieval and permeabilization methods should be optimized.
• As a polyclonal antibody, it may exhibit batch-to-batch variability; consider including appropriate controls and validating in the target species (human, mouse, rat).
• ELISA can be used for quantitative detection; pairing with a matched detection antibody may improve sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering a seamless procurement experience for this ASB3 antibody and related reagents. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets like ubiquitin-proteasome pathway components. Whether you need this product for routine assays or are developing novel diagnostic tests, CamelBio supports your entire workflow from concept to clinic.