Anti-ASB2 Rabbit Polyclonal Antibody for WB and ELISA - Q96Q27

Core Product Specifications and Parameters

Biological Background: ASB2 Function and Localization

• ASB2 functions as a substrate-recognition component of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins. (PubMed:15590664, PubMed:16325183) Related references: PMID:15590664 PMID:16325183
• It participates in Notch-induced signaling by promoting ubiquitination and degradation of substrates such as TCF3/E2A and JAK2 (PubMed:21119685). During embryonic development, ASB2 is crucial for complete heart looping and cardiomyocyte differentiation (PubMed:32179481). Related references: PMID:21119685 PMID:32179481
• ASB2 specifically targets SMAD9 for proteasomal degradation, regulating its cellular abundance to prevent excessive accumulation (PubMed:34845242). Related references: PMID:34845242
• In myogenic differentiation, ASB2 targets filamin FLNB and the intermediate filament protein DES for degradation (PubMed:19300455), acting as a negative regulator of skeletal muscle mass. Related references: PMID:19300455
• ASB2 mediates the degradation of filamins FLNA and FLNB (PubMed:21737450; PubMed:22916308; PubMed:24044920), enhancing hematopoietic cell adhesion to fibronectin (PubMed:21737450) and facilitating dendritic cell migration. In hematopoietic cells, ASB2 does not promote degradation of JAK1 or JAK2 (PubMed:22916308). Related references: PMID:21737450 PMID:22916308 PMID:24044920
• Subcellularly, ASB2 localizes to the cytoplasm, associating with cytoskeletal structures such as stress fibers and the sarcomeric Z-line in myofibrils.
• ASB2 is predominantly expressed in muscle cells and hematopoietic cells.
• The protein contains ankyrin repeat domains and a SOCS box, characteristic of the ASB family, and undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 448–587) of human ASB2, which may provide specificity for full-length ASB2. Cross-reactivity with rat suggests conserved epitopes.
• This polyclonal antibody is applicable for Western blotting (WB) and ELISA. For WB, consider using whole-cell lysates from human or rat muscle/hematopoietic cell lines, and validate band specificity around the predicted molecular weight of 70 kDa.
• Optimize primary antibody dilutions and incubation conditions empirically for your specific assay and sample type. Include appropriate positive and negative controls.
• If using for ELISA, assess coating antigen and detection conditions to minimize background and maximize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, from concept to clinic, supporting diagnostic manufacturers and research laboratories worldwide. This anti-ASB2 antibody serves the ubiquitin-proteasome and cardiovascular/hematology research fields. Our portfolio includes validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets—ensuring seamless procurement for your assay development and production needs.