Anti-ASB17 Polyclonal Antibody for WB, ELISA - Q8VHP9

Core Product Specifications and Parameters

Biological Background: ASB17 Function and Localization

• ASB17 (Ankyrin repeat and SOCS box protein 17) is a member of the ASB protein family, containing N-terminal ankyrin repeats and a C-terminal SOCS box domain.
• It is predicted to function as a substrate-recognition component of an SCF-like ECS (Elongin Cullin SOCS-box) E3 ubiquitin-protein ligase complex, facilitating ubiquitination and subsequent proteasomal degradation of target proteins.
• ASB17 is specifically expressed in the testis, predominantly in spermatogenic cells. Expression is highest in round and condensing spermatids, with lower levels detected in pachytene spermatocytes. Related references: PMID:15460110 PMID:15204681
• The protein is implicated in the ubiquitin conjugation pathway (Ubl conjugation pathway), consistent with its predicted role in protein ubiquitination.
• The mouse ASB17 protein has a calculated molecular weight of approximately 34.5 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the central region (aa 78-177) of mouse ASB17. This region lies within the ankyrin repeat domain, which may be conserved across species, resulting in the observed cross-reactivity with human and rat.
• For western blotting, consider that the predicted molecular weight of ASB17 is approximately 34 kDa. Post-translational modifications or cell-type-specific isoforms may result in additional bands; validate using the appropriate positive control (e.g., testis lysates from human or rat).
• In ELISA applications, ensure that the coating antigen and detection system are optimized, as performance may vary depending on the assay format and sample matrix.

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CamelBio offers comprehensive access to IVD raw materials, supporting researchers and diagnostic developers with high-quality antibodies like this anti-ASB17 polyclonal antibody. As a one-stop sourcing partner, we provide validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare target materials for specialized applications such as ubiquitin pathway and reproductive biology research.