Anti-ASB13 Polyclonal Antibody for WB, ELISA - Q8WXK3

Core Product Specifications and Parameters

Biological Background: ASB13 Function and Localization

• ASB13 (Ankyrin repeat and SOCS box protein 13) is a member of the ankyrin repeat and SOCS box-containing (ASB) protein family.
• It contains N-terminal ankyrin repeats that mediate protein–protein interactions and a C-terminal SOCS box domain responsible for recruiting the Elongin BC complex.
• ASB13 acts as a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex.
• This complex facilitates the ubiquitination of target proteins, targeting them for proteasomal degradation and thereby regulating protein turnover.
• The gene undergoes alternative splicing, generating multiple transcript variants (UniProt keyword: Alternative splicing).
• ASB13 has been identified in large-scale proteomics screens, consistent with its keyword annotation "Proteomics identification".
• The predicted molecular weight is approximately 30 kDa, aligning with the band observed in western blotting.

Experimental Guidance and Technical Tips

• For western blotting, a dilution range of 1:500–1:2000 is typical for rabbit polyclonals; optimise based on target abundance in your sample.
• The expected band size is ~30 kDa; use a 10–12% SDS-PAGE gel and ensure efficient transfer conditions.
• When working with mouse or rat tissues, include species-appropriate positive and negative controls to verify cross-reactivity.
• For ELISA, consider using the recombinant immunogen as a coating antigen to confirm antibody binding specificity.
• Since ASB13 functions within an E3 ubiquitin ligase complex, co-immunoprecipitation followed by western blotting could be employed to explore protein interactions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents tailored for ubiquitin-pathway research. This ASB13 polyclonal antibody is part of our portfolio for protein degradation studies; our team can additionally source or custom-develop reagents to meet your specific assay development requirements.