Anti-ASAP2 Polyclonal Antibody for WB - O43150

Core Product Specifications and Parameters

Biological Background: ASAP2 Function and Localization

• ASAP2, also known as DDEF2 or PAG3, is an Arf-GAP with SH3 domain, ANK repeat, and PH domain-containing protein 2.
• It activates the small GTPases ARF1, ARF5, and ARF6, thereby regulating post-Golgi vesicle formation and constitutive secretion.
• ASAP2 modulates phagocytosis mediated by Fc gamma receptor and ARF6, and influences paxillin (PXN) recruitment to focal contacts and cell migration.
• Subcellularly, ASAP2 localizes to the cytoplasm, Golgi apparatus (especially the Golgi stack membrane), and the cell membrane.
• Tissue expression includes heart, brain, placenta, kidney, monocytes, and pancreas.
• The protein contains multiple structural domains: ANK repeats, a coiled coil region, a PH domain, an SH3 domain, and a zinc finger motif.
• Post-translational modifications include phosphorylation; it is classified as a phosphoprotein involved in GTPase activation.
• Alternative splicing generates multiple isoforms of ASAP2.

Experimental Guidance and Technical Tips

• The immunogen targets the N‑terminal region (amino acids 1‑120) of human ASAP2, which may recognize distinct isoforms or proteolytic fragments; consider epitope conservation in your experimental system.
• For Western blotting, the expected molecular weight is approximately 112 kDa; validate detection in human and mouse lysates and optimize sample preparation and loading controls.
• For ELISA applications, consider indirect or sandwich formats and perform antibody titration to determine optimal coating and detection conditions.
• Cross-reactivity with mouse is reported; verify consistency in your specific mouse strain and tissue type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to validated IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This antibody targets ASAP2, a central regulator of Golgi‑to‑plasma membrane trafficking and cell migration, making it valuable for cell biology and signaling research. Through CamelBio, you can reliably source optimized polyclonal antibodies, complementary antibody pairs, bulk ancillary reagents, and even rare‑target raw materials to accelerate your assay development and production workflows.