Anti-ASAH2 Rabbit Polyclonal Antibody for WB, ELISA - Q9NR71

Core Product Specifications and Parameters

Biological Background: Neutral Ceramidase (ASAH2) Function and Localization

• ASAH2 encodes neutral ceramidase, a member of the ceramidase family, also known as BCDase, LCDase, N-CDase, and HNAC1.
• Functions as a plasma membrane ceramidase that hydrolyzes ceramides into sphingosine and free fatty acids at neutral pH, and can also catalyze the reverse synthesis reaction. Related references: PMID:10781606, PMID:16229686, PMID:26190575, PMID:11278489, PMID:17475390
• Together with sphingomyelinase, participates in the degradation of sphingomyelin to produce the bioactive lipids sphingosine and sphingosine-1-phosphate. Related references: PMID:16061940
• Plays a role in the digestion of dietary sphingolipids in the intestinal tract (by similarity).
• Ceramides, sphingosine, and sphingosine-1-phosphate regulate key cellular processes such as proliferation, apoptosis, and differentiation. Related references: PMID:15946935, PMID:19345744, PMID:24798654
• Subcellular localization includes the plasma membrane (rafts/caveolae), Golgi apparatus, mitochondrion, and secretion into the extracellular space (exosomes).
• Primarily expressed in the intestine, with additional expression in kidney, skeletal muscle, and heart; note that ubiquitous detection may be influenced by the paralog ASAH2B. Related references: PMID:17334805, PMID:10781606
• Contains structural features such as disulfide bonds, N‑glycosylation sites, a zinc‑binding domain, and phosphorylation/ubiquitination sites, reflecting its regulation and membrane association.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 80–340, a region within the extracellular domain of ASAH2. For Western blot (WB), a band of approximately 86 kDa is expected; reducing and denaturing conditions are recommended to resolve the glycosylated protein accurately.
• For ELISA applications, consider using the recombinant protein immunogen as a positive control and optimize coating conditions and detection reagents for best signal-to-noise ratio.
• Given the polyclonal nature, lot-to-lot consistency should be verified, and the antibody may recognize multiple isoforms or cleavage products—validate in relevant human, mouse, or rat tissue or cell lysates.
• Sphingolipid-enriched samples (e.g., intestinal or kidney tissue) may provide optimal endogenous detection; however, cross-absorption or blocking steps may be necessary to reduce non-specific binding in complex matrices.

CamelBio: Your One-Stop Sourcing Bridge

As a leading one-stop sourcing platform for IVD raw materials, CamelBio offers reliable access to validated antibody pairs, optimized polyclonal antibodies such as anti-ASAH2, and bulk ancillary reagents for sphingolipid metabolism and apoptosis research. Whether you need rare-target raw materials or technical support from concept to clinic, our integrated supply chain ensures quality and consistency for diagnostic development.