Anti-ASAH1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13510

Core Product Specifications and Parameters

Biological Background: ASAH1 (Acid Ceramidase) Function and Localization

• ASAH1 encodes a lysosomal enzyme that hydrolyzes ceramides into sphingosine and free fatty acids at acidic pH, with higher catalytic efficiency toward C12-ceramides (PubMed:10610716, PubMed:15655246, PubMed:7744740). Related references: PMID:10610716 PMID:15655246 PMID:7744740
• The enzyme also catalyzes the reverse reaction, synthesizing ceramides from sphingosine and fatty acids, with preference for D-erythro sphingosine and C12 or C14 fatty acids (PubMed:12764132, PubMed:12815059). Related references: PMID:12764132 PMID:12815059
• ASAH1 also exhibits N-acylethanolamine hydrolase activity, contributing to the regulation of bioactive lipid signaling molecules (PubMed:15655246). Related references: PMID:15655246
• By regulating ceramide, sphingosine, and sphingosine-1-phosphate levels in the epidermis, ASAH1 mediates calcium-induced differentiation of epidermal keratinocytes (PubMed:17713573). Related references: PMID:17713573
• ASAH1 indirectly regulates tumor necrosis factor (TNF)-induced apoptosis through ceramide and sphingosine balance (By similarity).
• In adrenocortical cells, ASAH1 may directly regulate steroidogenesis by binding the nuclear receptor NR5A1 and negatively regulating its transcriptional activity (PubMed:22261821). Related references: PMID:22261821
• Subcellular localization includes the lysosome, but ASAH1 can also be found in the nucleus, cytoplasm, and secreted.
• ASAH1 is broadly expressed with higher expression in the heart.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 140–389) covers a large portion of the mature acid ceramidase sequence; this design may yield antibodies recognizing both the full-length precursor and processed forms. Consider validating detection of isoforms in your sample system.
• For Western blot, the predicted molecular weight is approximately 45 kDa, but processing can generate smaller fragments. Use reducing conditions and verify band patterns with appropriate controls, especially in human, mouse, or rat lysates.
• In IF/ICC experiments, optimal fixation and permeabilization conditions should be determined to preserve lysosomal localization. Co-staining with organelle markers may help confirm specificity.
• When using this antibody in ELISA, consider its polyclonal nature for capture or detection reagent; pairing with a monoclonal antibody may improve signal-to-noise ratio. Optimize coating and blocking conditions for your specific assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop provider of IVD raw materials, offering a broad portfolio of validated antibody pairs and optimized polyclonal antibodies like this anti-ASAH1 reagent to support diagnostic assay development for sphingolipid metabolism and lysosomal storage disorders. Our sourcing capabilities extend to bulk ancillary reagents and rare target raw materials, helping streamline your workflow from concept to clinic. Whether you need high-quality antibodies for metabolic research or custom logistics solutions, CamelBio bridges your supply needs with reliable, B2B-focused service.