Anti-ARX Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96QS3

Core Product Specifications and Parameters

Biological Background: ARX Function and Localization

• ARX (Aristaless-related homeobox) is a homeobox-containing transcription factor that binds DNA at the consensus motif 5'-TAATTA-3' (PubMed:22194193, PubMed:31691806). Related references: PMID:22194193 PMID:31691806

• It is essential for brain development, regulating neuronal proliferation, interneuron migration, and differentiation in the embryonic forebrain (PubMed:11889467, PubMed:12379852, PubMed:14722918). Related references: PMID:11889467 PMID:12379852 PMID:14722918

• ARX directs pancreatic islet cell fate by promoting differentiation of glucagon-secreting alpha cells (By similarity).

• It also controls intestinal enteroendocrine cell differentiation into multiple hormone-producing subtypes (PubMed:37883554). Related references: PMID:37883554

• ARX localizes to the nucleus and associates with chromatin, consistent with its role as a sequence-specific DNA-binding regulator.

• Expression of ARX is highest in fetal and adult brain (telencephalon and ventral thalamus) and skeletal muscle; it is absent from the cerebellum.

• ARX collaborates with histone demethylase PHF8 to activate genes such as KDM5C, possibly through H3K4me3-enriched regulatory elements (PubMed:31691806). Related references: PMID:31691806

• Mutations in ARX are associated with X-linked intellectual disability, epilepsy, and lissencephaly, reflecting its critical neurodevelopmental roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the C‑terminal region (aa 500–end) of human ARX; therefore, the antibody may preferentially recognize epitopes in this domain.
• For Western blot, a band at approximately 58 kDa is anticipated in human, mouse, and rat samples. Validate specificity using appropriate positive and negative controls (e.g., ARX‑overexpressing lysates or knockout tissue).
• In IHC‑P and IF/ICC, expect predominantly nuclear staining due to ARX’s role as a transcription factor. Use normal brain or skeletal muscle sections as positive control tissues, and confirm by colocalization with a nuclear marker.
• ELISA performance can be assessed by coating plates with the immunizing peptide or recombinant ARX protein; titration experiments are recommended to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials and technical support. This anti-ARX polyclonal antibody is one of many validated neurodevelopmental and endocrinology reagents available through our sourcing network. We offer bulk polyclonal and monoclonal antibodies, matched antigen standards, and customized procurement for rare targets to accelerate your assay development.