Anti-ARVCF Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O00192

Core Product Specifications and Parameters

Biological Background: ARVCF Function and Localization

• Protein identity: Splicing regulator ARVCF, also known as Armadillo repeat protein deleted in velo-cardio-facial syndrome.
• Core function: Contributes to the regulation of alternative splicing of pre-mRNAs.
• Subcellular localization: Found at cell junctions (adherens junctions), as well as in the nucleus and cytoplasm.
• Tissue specificity: Expressed widely in heart, brain, liver, kidney, and at low levels in lung. High expression in epithelial cells, with lower levels in fibroblasts and T lymphocytes.
• Post-translational modifications: Subject to phosphorylation and methylation.
• Structural features: Contains armadillo-like repeats and a coiled-coil domain.
• Cell adhesion role: Localization to adherens junctions suggests a function in cell adhesion.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the expected molecular weight is approximately 105 kDa. Use appropriate protein ladders and transfer conditions to resolve this high-molecular-weight band.
• The immunogen is an N-terminal synthetic peptide (aa 1-100); thus, the antibody may recognize linear epitopes. Standard blocking agents (e.g., 5% non-fat milk or BSA) are typically suitable.
• For immunofluorescence (IF/ICC), consider optimization of fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) to preserve adherens junction and nuclear staining.
• In ELISA applications, the antibody can be employed for peptide or protein capture/detection; validate with the specific immunogen or recombinant protein.
• Cross-reactivity with mouse and rat has been reported; verify reactivity in your specific model system, as sequence homology may influence sensitivity.
• As with all antibodies, we recommend titration experiments within each assay system to determine the optimal working dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. For studies focused on ARVCF, alternative splicing, and cell adhesion, we provide validated polyclonal antibodies and can facilitate sourcing of matched pairs, recombinant proteins, and ancillary reagents. Our integrated supply chain and technical consultation help streamline your transition from concept to clinical application.