Anti-ARTN Rabbit Polyclonal Antibody for WB, ELISA - Q5T4W7

Core Product Specifications and Parameters

Biological Background: Artemin Function and Localization

• Artemin (ARTN), also named enovin or neublastin, is a secreted growth factor.
• It promotes the survival of sensory and sympathetic peripheral neurons in culture, as well as dopaminergic neurons of the ventral midbrain. Related references: PMID:10583383, PMID:9883723
• ARTN signals through binding to its coreceptor GFRA3 (GFRα3), which leads to autophosphorylation and activation of the RET receptor tyrosine kinase. Related references: PMID:31535977
• It acts as a strong chemoattractant for gut hematopoietic cells, promoting the formation of Peyer's patch-like structures in gut-associated lymphoid tissue.
• The protein is ubiquitously expressed, with high levels in peripheral tissues such as prostate, placenta, pancreas, heart, kidney, pituitary gland, lung, and testis, and low levels in the brain.
• Post-translational modifications include disulfide bonds and glycosylation, and multiple isoforms exist due to alternative splicing.
• ARTN has a molecular weight of approximately 23 kDa (calculated 22.9 kDa).

Experimental Guidance and Technical Tips

• For Western blotting, consider using this antibody in a dilution range appropriate for the target's molecular weight of 23 kDa; validate in lysates from relevant human, mouse, or rat tissues.
• For ELISA, the recombinant protein immunogen (amino acids 122-220) can serve as a coating antigen or positive control to establish assay conditions.
• Because the immunogen corresponds to a conserved region, the antibody may be suitable for detecting ARTN in multiple species; however, cross-reactivity should be confirmed in the specific experimental system.

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