Anti-ART3 Rabbit pAb for WB, IF-P, ELISA - Q13508

Core Product Specifications and Parameters

Biological Background: ART3 (Ecto-ADP-ribosyltransferase 3) Function and Localization

• ART3 encodes ecto-ADP-ribosyltransferase 3, also known as ARTC3, a mono(ADP-ribosyl)transferase that catalyzes the transfer of ADP-ribose from NAD+ to arginine residues of target proteins.
• The protein undergoes alternative splicing, producing multiple isoforms with potentially distinct regulatory or structural features.
• ART3 belongs to the ARTC (ADP-ribosyltransferase C2 and C3 toxin-like) family of enzymes, which share homology with bacterial toxins and are involved in post-translational modification.
• It is a cell membrane protein, anchored via a GPI (glycosylphosphatidylinositol) moiety, and contains a signal peptide, disulfide bonds, and glycosylation sites.
• Expression is testis-specific, suggesting a role in spermatogenesis or sperm function.
• Keywords: glycoprotein, transferase, NAD, GPI-anchor, alternative splicing, cell membrane, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 50-150 of human ART3, a region likely located extracellularly; ensure this matches the target epitope for your experimental system.
• For WB, consider using reducing conditions and appropriate controls to detect the 44 kDa protein, though post-translational modifications may cause band shifts.
• IF-P: optimize permeabilization and fixation to preserve cell membrane localization; include negative controls for specificity.
• ELISA: test cross-reactivity with rat samples if needed; validate sensitivity with recombinant protein standards.
• Note that ART3 is testis-specific, so select appropriate human or rat testis lysates/tissues for positive controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our ART3 antibody supports research in ADP-ribosylation and testis biology. We connect diagnostic manufacturers and labs with high-quality materials and technical services for streamlined development.