Anti-ART1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P52961

Core Product Specifications and Parameters

Biological Background: ART1 Function and Localization

• ART1 (ADP-ribosyltransferase 1) is a GPI-linked NAD(P)+-arginine ADP-ribosyltransferase, also designated ARTC1 or CD296.
• It belongs to the ADP-ribosyltransferase C2/C3 toxin-like family of mono(ADP-ribosyl)transferases that utilize NAD+ or NADP+ as substrates.
• ART1 catalyzes the transfer of ADP-ribose from NAD+ to arginine residues on target proteins; a known substrate is the glucagon-like peptide 1 receptor (GLP1R).
• The enzyme is anchored to the outer leaflet of the sarcoplasmic reticulum membrane via a C-terminal glycosylphosphatidylinositol (GPI) moiety.
• The primary translation product contains a signal peptide and undergoes N-linked glycosylation, disulfide bond formation, and GPI-attachment during secretion pathway processing.
• Key functional and structural features include its glycoprotein nature, GPI-anchor, lipid modification, and dependence on NAD/NADP cofactors.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 23–295 of human ART1, covering the N-terminal catalytic domain; this design supports high specificity for the mature protein.
• For western blotting, a band at approximately 36 kDa is expected (calculated molecular weight 36.3 kDa); validate detection using relevant human and mouse cell lines or tissues.
• In immunohistochemistry and immunofluorescence, optimize fixation and permeabilization protocols due to the membrane-associated GPI-anchored localization.
• The polyclonal nature may yield robust signal amplification; however, lot-to-lot consistency should be verified for quantitative applications.
• Detection across human and mouse species is documented; cross-reactivity with other organisms should be tested empirically if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner that connects diagnostic manufacturers, laboratories, and research institutes with high-quality IVD raw materials like the anti-ART1 antibody, ideal for investigating ADP-ribosylation and GLP1R signaling. Beyond this product, we support procurement of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for robust assay development. Our global network enables efficient sourcing of even rare-target raw materials, ensuring your projects progress smoothly from concept to clinic.