Anti-ARSA Polyclonal Antibody for WB and ELISA - P15289

Core Product Specifications and Parameters

Biological Background: ARSA Function and Localization

• ARSA encodes Arylsulfatase A, also known as cerebroside-sulfatase, a lysosomal enzyme.
• It catalyzes the hydrolysis of cerebroside-3-sulfate (sulfatide) into cerebroside and sulfate, a reaction requiring the activator protein saposin B.
• The protein localizes to the endoplasmic reticulum and lysosomes.
• Mutations in ARSA cause metachromatic leukodystrophy, a severe lysosomal storage disorder characterized by sulfatide accumulation.
• ARSA is a calcium-binding glycoprotein with disulfide bonds essential for its structure and enzymatic activity.
• Alternative splicing generates multiple isoforms, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 210–509, which may enable detection of multiple ARSA isoforms.
• For Western blot, consider using human or mouse tissue lysates as positive controls; a band near 54 kDa is expected.
• In ELISA, this polyclonal antibody may serve as a capture or detection reagent; optimization of conditions is advised.
• Cross-reactivity with human and mouse supports its use in translational models; validate specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This ARSA polyclonal antibody supports research into lysosomal storage disorders and lipid metabolism, and we can assist with sourcing rare-target materials to accelerate your diagnostic development from concept to clinic.