Anti-ARRDC4 Rabbit Polyclonal Antibody for WB, ELISA - Q8NCT1

Core Product Specifications and Parameters

Biological Background: ARRDC4 Function and Localization

• ARRDC4 is an arrestin domain-containing protein that functions as an adaptor recruiting ubiquitin-protein ligases to their specific substrates (By similarity).
• It plays a role in endocytosis of activated G protein-coupled receptors (GPCRs) through a ubiquitination-dependent mechanism (Probable).
• ARRDC4 is involved in the incorporation of SLC11A2 into extracellular vesicles via ubiquitination (By similarity).
• It may play a role in glucose uptake. Related references: PMID:19605364
• ARRDC4 participates in the innate immune response by promoting IFIH1/MDA5 activation through interaction with TRIM65. Related references: PMID:28594402
• Subcellular localization includes early endosomes, cell membrane, and cytoplasmic vesicles.
• Contains arrestin-like repeat domains and has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 300–400 of human ARRDC4, suggesting the antibody may detect an epitope in this region. Consider this when designing blocking peptides or epitope mapping experiments.
• For Western blot, a major band at approximately 45 kDa is expected. Validate specificity using positive (e.g., ARRDC4-expressing cell lines) and negative controls.
• In ELISA, the antibody can be used with the immunizing peptide as a coating antigen or to capture/detect native ARRDC4; optimize coating conditions and antibody dilutions empirically.
• Cross-reactivity is reported for human; if testing other species, sequence homology of the immunogen region should be assessed and validation performed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop platform for IVD raw materials, technical services, and consultative support—from concept to clinic. This ARRDC4 polyclonal antibody supports research focused on GPCR trafficking, glucose metabolism, and innate immune pathways. Our supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of raw materials for rare targets, helping streamline your assay development and production.