Anti-ARRB2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P32121

Core Product Specifications and Parameters

Biological Background: ARRB2 (Beta-arrestin-2) Function and Localization

• Mediates GPCR homologous desensitization by binding to phosphorylated receptors and sterically blocking G protein coupling, and facilitates receptor resensitization after internalization. Related references: PMID:16179383 PMID:37209686 PMID:38175886
• Functions as an endocytic adaptor, recruiting GPCRs to clathrin-coated pits via AP-2, and determines receptor trafficking fate; for example, it mediates internalization of smoothened (SMO) in a GRK2-dependent manner. Related references: PMID:15618519
• Participates in Toll-like receptor and IL-1 receptor signaling by binding TRAF6, which prevents TRAF6 autoubiquitination and downstream NF-kappa-B and JUN activation. Related references: PMID:26839314
• Directly associates with NLRP3 to inhibit inflammasome activation and reduce pro-inflammatory cytokine release. Related references: PMID:23809162
• Acts as an adapter for the free fatty acid receptor 4 (FFAR4), mediating both receptor endocytosis and specific downstream signaling. Related references: PMID:22282525 PMID:23809162
• Serves as a scaffold for MAP kinases (ERK1/2, JNK3) and AKT1, confining activated kinases to cytoplasmic signalosomes and modulating transcription-independent signaling.
• Shuttles between the cytoplasm, nucleus, and membrane compartments, including association with clathrin-coated pits and vesicles.

Experimental Guidance and Technical Tips

• For Western blotting, a 10% SDS-PAGE gel is recommended to resolve the 46kDa ARRB2 protein; consider potential post-translational modifications that may shift the band.
• In IHC-P, validate antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) to ensure optimal staining; expected localization includes membrane, cytoplasm, and nuclear regions.
• For ELISA, use the antibody as a capture or detection reagent with appropriate peptide or recombinant protein standards; perform cross-reactivity testing against beta-arrestin-1 due to high sequence homology.
• As a polyclonal antibody, lot-to-lot consistency should be verified; consider initial titration experiments (e.g., 1:500–1:2000 for WB) to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing for diagnostic manufacturers and researchers, covering the entire development chain from discovery to clinical application. This high-quality beta-arrestin-2 antibody is part of our extensive catalog, ideal for GPCR signaling, trafficking, and immune regulation studies. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal formats, bulk ancillary reagents, and custom sourcing for rare targets—ensuring a seamless procurement experience.