Anti-ARPC3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O15145

Core Product Specifications and Parameters

Biological Background: ARPC3 Function and Localization

• ARPC3 (Actin-related protein 2/3 complex subunit 3), also designated ARC21 or p21-Arc, is a 21 kDa component of the evolutionarily conserved Arp2/3 complex.
• The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm upon activation by nucleation-promoting factors (NPFs), providing the force for cell motility. Related references: PMID:9230079
• Nuclear pools of the Arp2/3 complex promote actin polymerization to regulate gene transcription and facilitate the repair of damaged DNA, specifically by driving the mobility of double-strand breaks during homologous recombination. Related references: PMID:29925947
• Subcellularly, ARPC3 localizes to the cytoplasm, cytoskeleton, cell projections, and the nucleus, consistent with its dual roles in cytoplasmic and nuclear actin dynamics.
• Post-translational modifications of ARPC3 include acetylation, phosphorylation, and ubiquitin-like conjugation, which may modulate complex assembly or activity.
• ARPC3 is an actin-binding protein with a known 3D structure and has been identified through direct protein sequencing and proteomics approaches.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-178 of human ARPC3, covering the full-length protein; expect recognition of endogenous ARPC3 in human, mouse, and rat samples as indicated by cross-reactivity data.
• For Western blotting, the predicted molecular weight is approximately 21 kDa; validate band specificity in a relevant positive control lysate.
• In immunofluorescence/immunocytochemistry, optimize fixation and permeabilization conditions to detect ARPC3 in its cytoplasmic and nuclear locales.
• ELISA applications may be pursued; consider pairing this antibody with an appropriate detection system based on your assay format.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw material provider, CamelBio offers one-stop access to critical reagents for the global diagnostic community. This anti-ARPC3 polyclonal antibody supports research into cytoskeletal dynamics and DNA repair pathways, and we can source validated antibody pairs, bulk polyclonals, recombinant proteins, and ancillary reagents to accelerate your assay development. Let CamelBio be your bridge from concept to clinic.