Anti-Arp2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P61160

Core Product Specifications and Parameters

Biological Background: Arp2 Function and Localization

• Arp2 (Actin-related protein 2), also known as Actin-like protein 2, is encoded by the ACTR2 gene.
• Arp2 is an ATP-binding component of the Arp2/3 complex, which mediates actin polymerization upon stimulation by nucleation-promoting factors, driving the formation of branched actin networks in the cytoplasm for cell motility. Related references: PMID:9000076
• In podocytes, Arp2 is required for lamellipodia formation downstream of AVIL and PLCE1 regulation. Related references: PMID:29058690
• The Arp2/3 complex also promotes actin polymerization in the nucleus, regulating gene transcription and repair of damaged DNA. Related references: PMID:17220302, PMID:29925947
• Nuclear Arp2/3 complex promotes homologous recombination repair by driving motility of double-strand breaks. Related references: PMID:29925947
• Arp2 localizes to the cytoplasm, cytoskeleton, cell projections, and nucleus.
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (aa 1-160) of human Arp2, which may help recognize both native and denatured protein conformations.
• For Western blotting, expect a band near 45 kDa; optimize loading controls and detection conditions.
• In immunohistochemistry and immunofluorescence, validate specificity using appropriate tissue/cell controls and consider signal localization patterns.
• Given reported cross-reactivity with mouse and rat, the antibody is suitable for comparative studies, but always confirm in the relevant model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, technical services, and consulting—from concept to clinic. For Arp2 and the Arp2/3 complex research, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist with sourcing rare target raw materials. Whether scaling up production or requiring tailored technical support, CamelBio bridges your sourcing needs with reliable supply chains.