Anti-Aromatase (CYP19A1) Rabbit Monoclonal Antibody for WB - P11511

Core Product Specifications and Parameters

Biological Background: Aromatase (CYP19A1) Function and Localization

• Aromatase (CYP19A1) is a cytochrome P450 monooxygenase that converts C19 androgens (androstenedione and testosterone) to C18 estrogens (estrone and estradiol) through three successive oxidations. Related references: PMID:27702664 PMID:2848247
• The enzyme catalyzes three successive oxidations: two conventional oxidations at C19 to 19-hydroxy and 19-oxo/19-aldehyde intermediates, then a third aromatization step resulting in phenolic A ring and formic acid, or alternatively producing a 19-norsteroid. Related references: PMID:20385561
• CYP19A1 also converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone, potentially regulating androgen homeostasis, and displays 2-hydroxylase activity toward estrone. Related references: PMID:22773874
• Subcellularly, aromatase localizes to the endoplasmic reticulum membrane and microsome membrane.
• The protein is widely expressed in tissues, including adult and fetal brain, placenta, skin fibroblasts, adipose tissue, and gonads.
• Keywords: alternative splicing, disease variant, heme and iron binding, lipid metabolism, transmembrane helix. Mutations in CYP19A1 are associated with pseudohermaphroditism.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human aromatase, which may provide specificity for intact CYP19A1.
• For Western blotting, a band at approximately 58 kDa is expected; consider using appropriate positive controls (e.g., placental tissue lysate) and validate the molecular weight in your system.
• Immunofluorescence and immunohistochemistry may reveal endoplasmic reticulum-like staining; co-localization with ER markers is recommended to confirm specificity.
• The antibody's reactivity with mouse and rat orthologs is listed; if using these species, verify sequence homology in the epitope region to ensure cross-reactivity.
• For ELISA applications, pair this antibody with a matched detection antibody or use it as a capture/detection reagent, and validate with recombinant aromatase or tissue extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for diagnostic raw materials, from concept to clinic. This anti-CYP19A1 antibody supports assays for estrogen biosynthesis, aromatase inhibitor development, and endocrine research. We supply validated antibody pairs, bulk ancillary reagents, and can source rare targets to accelerate your IVD project.