Anti-ARMET/ARP/MANF Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P55145

Core Product Specifications and Parameters

Biological Background: MANF (ARMET/ARP) Function and Localization

• Mesencephalic astrocyte-derived neurotrophic factor (MANF), also known as Arginine-rich protein (ARMET/ARP), is a secreted neurotrophic factor that promotes the survival of dopaminergic neurons and modulates GABAergic transmission in the substantia nigra. Related references: PMID:12794311
• MANF inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. It is retained in the ER/sarcoplasmic reticulum through association with the chaperone HSPA5/BiP under normal conditions and is secreted upon ER stress. Related references: PMID:18561914, PMID:22637475, PMID:29497057
• Secreted MANF binds to 3-O-sulfogalactosylceramide (sulfatide) on target cell membranes, promoting cellular uptake by endocytosis, which is required for its role in alleviating ER stress and cell toxicity under hypoxic conditions. Related references: PMID:29497057
• MANF is essential for embryonic lung development, postnatal splenic white pulp architecture, endochondral ossification, and skeletal muscle repair, where it regulates macrophage phenotypic transitions to limit inflammation.
• Subcellularly, MANF localizes to the endoplasmic reticulum lumen and sarcoplasmic reticulum lumen under normal conditions, and is secreted in response to stress.
• Post-translational modifications include glycosylation, disulfide bond formation, phosphorylation, and sialic acid addition. MANF functions in the unfolded protein response (UPR) and stress response pathways.
• Mutations in MANF are associated with deafness, diabetes mellitus, and dwarfism, underscoring its diverse physiological roles.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant fusion protein spanning amino acids 25-182 of human MANF, covering the mature polypeptide. This region is well-conserved across human, mouse, and rat, supporting cross-reactivity.
• Western blot (WB): The predicted molecular weight is 21 kDa; however, consider that post-translational modifications (e.g., glycosylation) may cause slight band shifts. Validate with appropriate positive controls and consider ER stress induction models to assess regulated secretion.
• Immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC): Due to MANF's secretion and ER localization, ensure adequate permeabilization and include ER markers for co-localization studies. Optimize antigen retrieval for paraffin-embedded sections.
• Immunoprecipitation (IP) and ELISA: The polyclonal nature provides robust capture and detection capacity. For sandwich ELISA, consider pairing with a monoclonal antibody recognizing a distinct epitope.
• General note: As a polyclonal antibody, lot-to-lot consistency should be verified in your specific assay system. Always include appropriate isotype and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk antibodies, and ancillary reagents. This anti-MANF polyclonal antibody supports neurotrophic factor and ER stress research, aligning with our commitment to advancing diagnostic and therapeutic development. Explore our one-stop sourcing services for difficult-to-find targets, customized antibody development, and technical consulting.