Anti-ARMET/ARP/MANF Rabbit Monoclonal Antibody for WB, ELISA - P55145

Core Product Specifications and Parameters

Biological Background: MANF Function and Localization

• MANF (mesencephalic astrocyte-derived neurotrophic factor), also known as ARMET (arginine-rich protein) or ARP, is a secreted protein that resides in the endoplasmic reticulum (ER) and sarcoplasmic reticulum lumen.
• Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain, playing a critical role in neuroprotection. Related references: PMID:12794311
• Modulates GABAergic transmission to dopaminergic neurons of the substantia nigra, enhancing both spontaneous and evoked inhibitory postsynaptic currents.
• Inhibits cell proliferation and protects against endoplasmic reticulum (ER) stress-induced cell death by stabilizing the ER chaperone HSPA5/BiP and suppressing the unfolded protein response (UPR). Related references: PMID:18561914 PMID:22637475 PMID:29497057
• Upregulated and secreted in response to ER stress and hypoxia; mediates cellular uptake via binding to the lipid sulfatide (3-O-sulfogalactosylceramide) on target cell membranes, which is required for alleviating ER stress and cytotoxicity. Related references: PMID:22637475 PMID:29497057
• Essential for postnatal development processes including lung maturation, splenic white pulp architecture, skeletal muscle repair, myocardial protection, and endochondral ossification.
• Key structural features include a signal peptide, disulfide bonds, glycosylation, and phosphorylation sites, with a mature molecular weight of approximately 20.7 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 25–182 of human MANF, covering the mature protein after signal peptide cleavage, which is conserved across human, mouse, and rat.
• Suitable for western blot (WB) under reducing conditions, where a band around 21 kDa is expected; consider optimizing antibody dilution and exposure time based on sample expression levels.
• For ELISA, both direct and sandwich formats may be explored; antigen coating conditions and detection antibody concentration should be validated in the relevant sample system.
• Cross-reactivity with mouse and rat allows for multispecies experimental designs; always include appropriate species-matched controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-MANF rabbit monoclonal antibody is part of our comprehensive portfolio for neuroscience and cellular stress research. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare target raw-material sourcing, we supply the tools to accelerate your diagnostic development.