Anti-ARL8B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NVJ2

Core Product Specifications and Parameters

Biological Background: ARL8B Function and Localization

• ARL8B (ADP-ribosylation factor-like protein 8B), also known as ARL10C or Gie1, is a small GTPase that cycles between an active GTP-bound and an inactive GDP-bound state, functioning as a molecular switch in intracellular trafficking. Related references: PMID:15331635, PMID:16537643

• It plays a central role in lysosomal positioning and motility by recruiting effector proteins: it binds PLEKHM2 to recruit kinesin-1, driving anterograde lysosomal transport, and interacts with PLEKHM1 and the HOPS complex subunit VPS41 to facilitate lysosomal fusion and cargo delivery. Related references: PMID:16537643, PMID:16650381, PMID:25898167, PMID:22172677, PMID:25908847

• ARL8B is critical for immune cell function: it drives polarization of cytolytic granules and the microtubule-organizing center toward the immune synapse in natural killer cells, enabling cytotoxicity, and regulates lysosomal exocytosis for antigen presentation via MHC II and CD1d, as well as microbial killing. Related references: PMID:24088571, PMID:21802320, PMID:29592961

• In neurons, ARL8B mediates anterograde axonal transport of presynaptic lysosome-related vesicles, which is essential for presynaptic biogenesis and synaptic function (By similarity).

• It also regulates endosome-to-lysosome trafficking and endosome-to-trans-Golgi network retrieval, with roles in recruiting RUFY1 to early endosomes. Related references: PMID:36282215

• ARL8B may participate in chromosome segregation during cell division. Related references: PMID:15331635

• During microbial infection, ARL8B is required for plasma membrane repair in macrophages via lysosomal exocytosis; it distinguishes virulent from avirulent Mycobacterium tuberculosis-induced necrotic cell death, and coronaviruses may exploit this pathway for egress.

• Subcellularly, ARL8B localizes to late endosomes, lysosomes, early endosomes, cytolytic granules, the mitotic spindle, and neuronal axons and synapses. It is ubiquitously expressed and undergoes post‑translational modifications including acetylation, isopeptide bonding, and ubiquitin‑like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 127–186 of human ARL8B (NP_060654.1), a central region of the protein; the antibody is expected to recognize full‑length ARL8B.
• For western blotting, a band at approximately 22 kDa should be observed; validate the antibody in your specific sample system and consider using appropriate positive controls (e.g., HeLa, Jurkat lysates).
• In immunofluorescence/immunocytochemistry, ARL8B localizes to lysosomal and endosomal compartments; include organelle‑specific markers and optimize permeabilization to confirm correct localization.
• For ELISA, the antibody can be used for detection or capture after appropriate pairing validation and titration.
• The antibody is reported to cross‑react with human and mouse ARL8B; if applying to other species, check sequence homology of the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, supporting diagnostic manufacturers and researchers from early development to clinical application. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for targets in intracellular trafficking and immune function, such as ARL8B. Leverage our sourcing expertise to accelerate your assay development and secure high-quality reagents for lysosomal biology and immunology research.