Anti-ARL6IP5 Rabbit Polyclonal Antibody for WB and ELISA - O75915

Core Product Specifications and Parameters

Biological Background: ARL6IP5 Function and Localization

• ARL6IP5, also known as PRA1 family protein 3, is a multi-pass membrane protein with aliases including JWA, PRAF3, GTRAP3-18, and DERP11.
• It negatively modulates the glutamate transporter SLC1A1/EAAC1 by decreasing its affinity for glutamate in a PKC activity-dependent manner, thereby regulating intracellular glutamate levels.
• ARL6IP5 plays a role in the retention of SLC1A1/EAAC1 in the endoplasmic reticulum, influencing transporter trafficking.
• It is involved in the regulation of intracellular taurine concentrations, contributing to osmolyte balance.
• Subcellularly, ARL6IP5 localizes to the endoplasmic reticulum membrane, cell membrane, cytoplasm, and cytoskeleton.
• The protein contains a predicted transmembrane helix and is subject to acetylation, as identified by proteomics and direct protein sequencing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide from amino acids 100 to the C-terminus of human ARL6IP5. When validating, consider epitope accessibility in the native protein.
• For Western blot, the expected molecular weight is approximately 22 kDa; consider using appropriate gel percentage and transfer conditions.
• Cross-reactivity with mouse ARL6IP5 is indicated, which may support studies in mouse models; however, verify species-specific reactivity in your experimental system.
• ELISA applications may benefit from optimization of coating antigen concentration and antibody dilution to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects focused on glutamate transport or neurological disorders, this anti-ARL6IP5 antibody can be part of a tailored sourcing package.
• Our platform supports diagnostic manufacturers and research labs worldwide with streamlined access to rare-target reagents and technical consulting, helping you accelerate development from concept to clinic.