Anti-ARL6IP4 Rabbit Polyclonal Antibody for IHC-P and ELISA - Q66PJ3

Core Product Specifications and Parameters

Biological Background: ARL6IP4 Function and Localization

• ADP-ribosylation factor-like protein 6-interacting protein 4 (ARL6IP4), also known as SRp25, SRrp37, SFRS20, SR-25, and HSP-975, is a nuclear phosphoprotein involved in pre-mRNA alternative splicing regulation.
• ARL6IP4 modulates splice site selection by either activating 5' distal sites or promoting preferential use of 3' proximal sites, thereby influencing alternative splicing outcomes.
• Upon Herpes simplex virus 1 (HSV-1) infection, ARL6IP4 may function as a splicing inhibitor, specifically suppressing viral pre-mRNA processing.
• The protein localizes predominantly to the nucleus, with enrichment in nucleoli and nuclear speckles, compartments associated with mRNA processing and splicing assembly.
• Tissue-specific expression studies reveal that isoforms 3 and 7 are prominently expressed in brain, pancreas, prostate, and testis, whereas other isoforms show low or undetectable levels in these tissues.
• ARL6IP4 is subject to post-translational modifications, including phosphorylation and ubiquitin-like conjugation, and undergoes RNA editing, highlighting its dynamic regulation.
• Keywords associated with ARL6IP4 include alternative splicing, mRNA processing, mRNA splicing, nucleus, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 313–402 of human ARL6IP4 (NP_057722.3), a C-terminal region; ensure the target epitope is present in your sample’s isoform, as splice variants may differ in this domain.
• For immunohistochemistry (IHC-P), consider antigen retrieval conditions and use appropriate positive/negative controls (e.g., tissues with known ARL6IP4 expression like testis or brain) to validate staining specificity.
• In ELISA, use the recombinant immunogen as a positive control; due to the polyclonal nature, expect broad reactivity but confirm specificity with recombinant target protein and relevant negative controls.
• Given the protein’s role in alternative splicing and the existence of multiple isoforms, we recommend validating the antibody’s performance in your specific cell line or tissue context, possibly by western blot (though not listed as a validated application, the antibody may work in denaturing conditions with optimization).

CamelBio: Your One-Stop Sourcing Bridge

As a trusted one-stop provider of IVD raw materials, CamelBio supports diagnostic manufacturers and research laboratories with reliable access to antibodies like anti-ARL6IP4, tailored for alternative splicing and viral host-factor studies. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and rare-target sourcing capabilities to streamline your assay development. Partner with CamelBio to accelerate your projects from concept to clinic with expert technical and supply support.