Anti-ARL6 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9H0F7

Core Product Specifications and Parameters

Biological Background: ARL6 Function and Localization

• ARL6 (ADP-ribosylation factor-like protein 6, also known as BBS3) is a small GTP-binding protein belonging to the ARF family.
• It functions in membrane protein trafficking at the ciliary base by recruiting the BBSome coat complex to the plasma membrane, a process essential for sorting proteins to primary cilia. Related references: PMID:20603001
• Together with BBS1 and the BBSome complex, ARL6 regulates trafficking of PKD1 (by similarity) and SMO to cilia, thereby modulating sonic hedgehog (SHH) signaling. Related references: PMID:22072986
• ARL6 is implicated in ciliary assembly/disassembly and Wnt signaling cascades. Related references: PMID:20207729
• It localizes to the cilium membrane, basal body, and axoneme, and is also present in the cytoplasm.
• Myristoylated and GTP-binding, ARL6 requires magnesium for its activity. Alternative splicing generates an isoform required for proper retinal function (by similarity). Mutations in ARL6 cause Bardet-Biedl syndrome 3, characterized by ciliopathy-related phenotypes including retinal degeneration, obesity, and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human ARL6 protein, suggesting potential recognition of both native and denatured conformations; consider testing in native versus denaturing conditions.
• For Western blot, the predicted molecular weight is approximately 21 kDa; validate with appropriate positive controls (e.g., ciliated cell lines) and consider possible post-translational modifications that may shift the band.
• IHC-P: ARL6 localizes to primary cilia; ensure fixation and antigen retrieval methods preserve ciliary structures. Include relevant controls.
• The antibody cross-reacts with mouse, enabling its use in mouse models of ciliopathies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, antigens, and ancillary reagents to support diagnostic development from concept to clinic. This anti-ARL6 antibody addresses research and assay needs in ciliopathy and membrane trafficking, and we can assist with sourcing bulk quantities, custom formulations, or complementary rare target reagents to streamline your workflow.