Anti-ARL4D Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P49703

Core Product Specifications and Parameters

Biological Background: ARL4D Function and Localization

• ARL4D (ADP-ribosylation factor-like protein 4D), also known as ARF4L, is a small GTP-binding protein belonging to the ADP-ribosylation factor (ARF) family of GTPases.
• It cycles between an inactive GDP-bound and an active GTP-bound state, with cycling regulated by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs).
• Unlike some ARF family members, ARL4D does not act as an allosteric activator of cholera toxin catalytic subunit.
• In its GDP-bound form, ARL4D recruits the cytohesin family members CYTH1, CYTH2, CYTH3, and CYTH4 to the plasma membrane.
• ARL4D localizes to the nucleus, nucleolus, cytoplasm, and cell membrane, indicating dynamic shuttling between cellular compartments.
• Post-translational modifications include myristoylation, which likely facilitates membrane association.
• ARL4D is a nucleotide-binding protein involved in intracellular trafficking and signaling.

Experimental Guidance and Technical Tips

• The antibody is generated against a recombinant protein spanning amino acids 92–201 of human ARL4D (NP_001652.2); this region may be conserved across the reported reactive species (human, mouse, rat).
• For Western blot (WB), a band around 22 kDa is expected; optimize loading and transfer conditions for detection.
• In immunohistochemistry (IHC-P), validate staining specificity with appropriate controls and consider antigen retrieval protocols for formalin-fixed paraffin-embedded tissues.
• For ELISA, pair with a suitable detection antibody and optimize coating conditions to ensure sensitivity.
• As a polyclonal antibody, lot-to-lot consistency may vary; it is advisable to titrate each new lot and confirm specificity in the experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This anti-ARL4D antibody supports research into GTPase signaling and membrane trafficking, and CamelBio can assist with sourcing rare-target raw materials and bulk orders for assay development. From concept to clinic, CamelBio streamlines your supply chain with comprehensive technical support and consulting services.